HCV Nonstructural protein 3 Proteins Research Reagents

Nonstructural protein 3 (NS3, also known as p-70), is a viral nonstructural protein that is a 70 kDa cleavage product of the hepatitis C virus (HCV) polyprotein. HCV NS3 displays three enzymatic activities: serine protease, NTPase, and RNA helicase. The C-terminal two-thirds of the NS3 protein contains a helicase and a nucleic acid-stimulated nucleoside triphosphatase (NTPase) activities which are probably involved in viral replication. The first (N-terminal) 180 aminoacids of NS3 have an additional role as cofactor domains for NS2 protein.

HCV Nonstructural protein 3 Protein (1)

    HCV Nonstructural protein 3 Background

    HCV NS3 displays three enzymatic activities: serine protease, NTPase, and RNA helicase. HCV NS3 serine protease, in association with NS4A, is responsible for the cleavages of NS3-NS4A, NS4A-NS4B, NS4B-NS5A, and NS5A-NS5B. HCV NS3 RNA helicase binds to RNA and unwinds dsRNA in the 3' to 5' direction, and likely RNA stable secondary structure in the template strand (By similarity). Cleaves and inhibits the host antiviral protein MAVS. NS3/NS4A complex also prevents phosphorylation of human IRF3, thus preventing the establishment of dsRNA induced antiviral state. One of the HCV proteases, NS3-4A serine protease, is a non-covalent heterodimer consisting of a catalytic subunit (the N-terminal one-third of NS3 protein) and an activating cofactor (NS4A protein) and is responsible for cleavage at four sites of the HCV polyprotein.

    HCV Nonstructural protein 3 References

    • Pomerantz J.L., et al.,(1999), NF-kB activation by a signaling complex containing TRAF2, TANK, and TBK1, a novel IKK-related kinase. EMBO J. 18:6694-6704.
    • Tojima Y., et al., (2000), NAK is an IkappaB kinase-activating kinase.Nature 404:778-782.
    • Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

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