The HA tag (amino acid sequence: YPYDVPDYA) is derived from the human influenza virus hemagglutinin (HA) protein corresponding to amino acids 98-106. It has been extensively used as a general epitope tag and has a molecular weight of 1.1 KDa. Anti-HA Tag antibodies facilitate the detection, isolation, and purification of HA-tagged target proteins without a protein-specific antibody or probe.
Sino Biological anti-HA Tag antibodies can successfully detect recombinant HA-tagged proteins, and every lot of our anti-HA Tag antibodies are fully validated for use in multiple applications, including ELISA, Western Blotting (WB), Flow Cytometry (FCM), Immunoprecipitation (IP) and Immonofluorecence (IF).
|Product description||Application||Catalog# (PDF)||Size||Price||Select|
|Anti-HA Tag Antibody, Mouse MAb||ELISA WB FCM IF IP ICC/IF||50 µL|
|Anti-HA Tag Antibody, Rabbit PAb, Antigen Affinity Purified||WB IP||100028-T36||50 µL|
|Anti-HA Tag Antibody, Mouse MAb||IP||50 µL|
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Key Features of Anti-HA Tag Antibodies:
• Recognizes HA epitope tag at N terminus or C terminus
• High sensitivity, higher than most brands
• Multiple applications, satisfy all your research needs
Flow cytometry staining of HA Tag in 293 cells, transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D) using purified mouse Anti-HA Tag antibody (Cat No. 100028-MM10).
Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 30 min on ice. Cells were washed twice and analyzed by flow cytometry.
| Immunofluorescence staining of HA-Tag in 293 cells, transfected with HA-ARG1 (Figure A), HA-mFABP4 (Figure B), ARG1-HA (Figure C) and mFABP4-HA (Figure D) using anti-HA tag antibody (up: Cat: 100028-MM10; down: from Competitor A).
Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-HA-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
Compatible Secondary Antibodies:
> Goat Anti-Mouse IgG-Fc Secondary Antibody (HRP) (Cat.no: SSA020)
> Goat Anti-Rabbit IgG Secondary Antibody (HRP) (Cat.no: SSA004)
> Goat Anti-Rabbit IgG Secondary Antibody (Biotin) (Cat.no: SSA011)
> Goat Anti-Mouse IgG-Fc Secondary Antibody (Biotin) (Cat.no: SSA013)