GST Proteins Research Reagents

Glutathione S-transferase (GST) catalyzes the formation of glutathione S-conjugates between GSH and certain electrophilic substrates. It plays an important role in the biotransformation of many xenobiotics. GST may also function as a storage protein or ligandin for parasitotoxic ferriprotoporphyrin IX (hemin). GST is an abundantly expressed 26 kDa eukaryotic protein. It is an N-terminal fusion protein that has been proven to promote solubility and expression. GST is one of the first widely adopted fusion tags for affinity purification and has been used to purify innumerable proteins.

GST Protein (2)

GST Lysate (1)

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    GST Background

    Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 22amino acids(roughly 26 KDa), which, compared to other tags like the Myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include athrombindomain for cleavage of the GST tag during protein purification.

    GST References

      • Douglas KT . et al., 1987, Adv Enzymol Relat Areas Mol Biol.?59: 103- 67.?
      • Beckett GJ. et al., 1993, ?Adv Clin Chem. 30: 281-380.
      • Wilce MC. et al., 1994, ?Biochim Biophys Acta. 1205?(1): 1-18.
      • Leaver MJ. et al., 1998, ?Marine Environmental Research. 46?(1-5): 71-4.

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