What is Good Result for Nuclear Protein in Immunocytochemistry (ICC)?

It is more difficult to confirm the immune fluorescence result if the target molecules or proteins expressed in nucleus because the positive result has registration with the DAPI result of nucleus. Certainly, the result is also can be read clearly because the color of the positive result is different with the DAPI results. Here show the good and bad immune fluorescence result to explain how to give the conclusion.

Good Result

Target:Human RPS6KA2
Bad Results

Target:Human GNL3