Green fluorescent protein (GFP) derived from the jellyfish Aequorea victoria has become an important marker for visualization of protein localization, monitoring physiological processes and for detecting transgenic or transient protein expression in vivo. The GFP tag contains 238 amino acids, and has a molecular weight of 26.9 KDa. GFP can be used for the generation of fusion proteins without significantly interfering with native protein assembly and function, which makes this tag a very powerful tool for in vivo analyses.
Sino Biological anti-GFP Tag antibodies can reliably detect GFP (Green fluorescent protein), and every lot of our anti-GFP Tag antibodies are fully validated in various applications, including Western Blotting (WB), ELISA and Immunoprecipitation (IP).
|Product description||Application||Catalog# (PDF)||Size||Price||Select|
|GFP Tag Antibody, Rabbit PAb||ELISA||100 µL|
|GFP Tag Antibody, Rabbit MAb||WB ELISA IP||50 µL|
|GFP Tag Antibody, Rabbit PAb, Antigen Affinity Purified||ELISA||50 µL|
|*Please tick the checkbox, and add the Anti-GFP Antibody of interest to cart.||Add to Cart|
|GFP tag was immunoprecipitated using:
Lane A: 0.5 mg GFP transfected E.coli Lysate
Lane B: 0.5 mg GFP transfected 293 Cell Lysate
2 µL anti-GFP tag rabbit monoclonal antibody (Cat: 13105-R208) and 15 μl of 50 % Protein G agarose.
Primary antibody: Anti-GFP tag rabbit monoclonal antibody, at 1/1000 -1/5000 dilution
Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1/5000 dilution
|Immunoprecipitation - Anti-GFP Tag Antibody|
Gao S, Wang J, Zhang T, Liu G, Jin L, Ji D, Wang P, Meng Q, Zhu Y, Yu R. Low expression of CAPON in glioma contributes to cell proliferation via the Akt signaling pathway. International journal of molecular sciences. 2016 Nov 18;17(11):1859.
Application: Western Blot (WB)
Compatible Secondary Antibodies:
> Goat Anti-Rabbit IgG Secondary Antibody (Biotin) (Cat: SSA011)
> Goat Anti-Rabbit IgG-Fc Secondary Antibody (Biotin) (Cat: SSA010)
> Goat Anti-Rabbit IgG-Fc Secondary Antibody (HRP) (Cat: SSA003)
> Goat Anti-Rabbit IgG Secondary Antibody (HRP) (Cat: SSA004)
Immunoprecipitation / IP Kit:
> GFP Tag Immunomagnetic Beads(Cat: TB13105)