Strictly Validated Antibodies
| Verified by Multiple Cell Samples With an antibody validation through multiple cell lines will further clarify the antibody specificity. |
| Antigen Blocking Verified This method is one of the most conventional antibody specificity verification methods. |
| Non-conjugated Antibody Blocking Verified One purpose of this verification experiment with unlabeled antibodies is qualifying whether the specificity change after the labelling. |
| Fc Receptor Blocking Verified It's always be recommended to do Fc receptors blockade prior to sample staining if your sample contains some special cell types. |
| WB,IF and IHC Verified Other applications also provide information on specificity of the antibody. |
Comparable with Classic Clone
The vast majority of monoclonal antibodies were comparable to the classic clones, which had good reputation internationally, in homogeneity, specificity, sensitivity, affinity, etc..
Excellent Performance in sensitivity
The antibody products in Sino Biological Inc. (SBI) have high affinity and sensitivity performance. They could be effective in very high dilution to recommended concentration in your experiments.
Verified High Stability
Antibody stability in Sino Biological Inc. (SBI) produced reagents is determined by real-time and real-temperature testing. We can certify the period of time that the antibody has been tested.
So far, there are over 1500 strictly validated antibodies developed in Sino Biological Inc. favorable for Flow Cytometry assay.
Recommendations for Background Control
- Unlabeled cells as blank control
- Single color and FMO control
- Isotype control
- Negative cells control
Fluorochrome Selection
- Excitation and Emission
- Fluorochrome Selection
Flow Cytometry Tips
- Sample preparation
- Fluorescence staining
Flow Cytometry FAQ
- What fluorochromes can I use?
- What controls do I need?
- Do the cells need to be permeabilized?
- Do the samples need to be fixed?
Flow Cytometry Troubleshooting
- No staining
- Weak staining
- Nonspecific staining
- Unexpected staining
Flow Cytometry / FACS Background
Flow cytometry is a method to evaluate cell membrane proteins and intracellular proteins as well as peptides and DNA. The principle behind FACS is an antigen-antibody reaction, with the antibodies being fluorescently labelled. There are three fluorescent proteins (R-PE, APC, and PerCP) conjugated to antibodies. Flow cytometry quantification is carried out with intercalating color labels (without the antibody). Flow cytometry antibodies are widely used in cell counting, cell sorting, biomarker detection and protein engineering.
Antibody
- • Flow Cytometry (FCM) / FACS Antibody
- - Flow Cytometry (FCM) / FACS Antibody Features
- - Flow Cytometry (FCM) / FACS Products Center
- - Flow Cytometry (FCM) / FACS Technology Center
- • SARS-CoV-2 Antibody
- • ELISA Antibody
- • Immunoprecipitation / IP Antibody
- • Immunohistochemistry / IHC Antibody
- • Flow Cytometry (FCM) / FACS Antibody
- • Western blotting / WB Antibody
- • Immunofluorescence / IF / ICC Antibody
- • Tag Antibodies
- • Secondary Antibodies
- • Control Antibodies
- • Loading Control antibodies
- • Mouse Mab
- • Rabbit Mab
- • Rabbit Pab
- • Biomarker Antibody
- • CD Molecule Antibody
- • Stem Cell Research Antibody
- • Receptor Antibody
- • Interleukin Antibody
- • Cytokine Antibody
- • Biological Target Antibody
- • Diagnostic Antibody
- • Cancer Antibody
