Expression-ready ORF Clone

Sino Biological provides ready-to-use expression ORF clones without subcloning. The expression ORF clones can be use for transfecting immediately. Now thousands of molecules have been expression validated. Sino Biological has always been committed to providing customers with high quality products and services.

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Advantages of Expression-ready ORF Clone

Multiple Species
Human, mouse, rat, monkey, canine, ferret and virus...
Multiple Tags
• Untagged • C-terminal：Myc, HA, FLAG, His, GFPSpark® and OFPSpark® • N-terminal：Myc, HA, FLAG, His, GFPSpark® and OFPSpark®
Full Length Sequence Confirmed
Full length sequence confirmed, quality assurance

Expression Validated

Expression validated, high expression level

Applicability

Designed for mammalian expression, stable or transient

High Expression Level

Enhanced CMV promoter

Expression-ready ORF Clone: Higher protein expression level

The plasmid was transfected into mammalian cells with Sinofection reagent (Cat#STF02) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. EGFP protein expression levels were significantly increased in different cell lines.

pCMV3-EGFP(Sino Biological):

pCDNA3.1-EGFP(Other company):

Expression-ready ORF Clone: Expression validated

• Sino Biological provides ready-to-use expression ORF clones without subcloning. The expression ORF clones can be use for transfecting immediately. Now thousands of molecules have been expression validated.
• The Myc, HA, FLAG, His tagged ORF clones were expression validated by IF (Immunofluorescence). The plasmids were transfected into mammalian cells. After 48h, cells were fixed and blocked, incubated with primary antibodies, then stained with secondary antibody. Finally the fluorescent signals can be detected by fluorescence microscope.
• The GFPSpark® & OFPSpark® tagged ORF clones were transfected into cells transiently. After 48h, the fluorescent signals can be detected by fluorescence microscopy.

Human PTPN1, N-Flag
(HG10304-NF)

Human IDH1, N-GFPSpark®
(HG12055-ANG)

Human SIRT6, C-GFPSpark®
(HG12156-ACG)

H7N9-NP, C-Flag
(VG40110-CF)

Human PKM2, C-OFPSpark®
(HG11430-ACR)

Human MTOR, C-OFPSpark®
(HG11508-ACR)

Expression-ready ORF Clone by Tags

Untagged cDNA ORF Clone	N / C-FLAG Tag cDNA ORF Clone
N / C-Myc Tag cDNA ORF Clone	N / C-His Tag cDNA ORF Clone
N / C-HA Tag cDNA ORF Clone	N / C-GFPSpark® Tag cDNA ORF Clone
N / C-OFPSpark® Tag cDNA ORF Clone

Partly Expression Validated ORF Clones

Catalog No.	Molecule	Product Description	Availability
HG10304-NF	PTPN1	Human PTP1B / PTPN1 Gene ORF cDNA clone expression plasmid, N-Flag tag	In stock
HG11430-ACR	PKM	Human PKM2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag	In stock
HG11508-ACR	MTOR	Human MTOR Gene ORF cDNA clone expression plasmid, C-OFPSpark tag	In stock
HG12054-ACG	RELA	Human RELA / Transcription factor p65 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag	In stock
HG12055-ANG	IDH1	Human IDH1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tag	In stock
HG12125-NF	FTO	Human Fat mass and obesity associated/FTO Gene ORF cDNA clone expression plasmid, N-Flag tag	In stock
HG12156-ACG	SIRT6	Human SIRT6 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag	In stock
HG12283-CF	CD47	Human CD47 Gene ORF cDNA clone expression plasmid, C-Flag tag	In stock
MG50843-CF	CXCR5	Mouse CXCR5 Gene ORF cDNA clone expression plasmid, C-Flag tag	In stock
RG80100-NF	EGFR	Rat EGFR/HER1 Gene ORF cDNA clone expression plasmid, N-Flag tag	In stock
VG40042-CF	RSV-F	Human respiratory syncytial virus (RSV) (subtype A, strain A2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-Flag tag	In stock
VG40110-CF	H7N9-NP	Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag	In stock

Organelle Marker Plasmid-GFPSpark® /OFPSpark® tagged-expression ready ORF Clones

Sino Biological provides a collection of organelle marker plasmids which encoding organelle-specific or structure-specific proteins. The proteins are fused with GFPSpark® or OFPSpark® tag and allow direct visualization of the organelles or structures without manipulation by fluorescence microscopy. Organelle marker plasmids represent a part of expression ORF clone. Learn more about...
The GFPSpark® & OFPSpark® tag ORF clones were transfected into Hela cells transiently. After 48h, the fluorescent signals can be detected by laser scanning confocal microscope (Nikon) (GFPSpark® excitation/ emission max = 487 / 508 nm, OFPSpark® excitation/emission maxima =549 / 566 nm).

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