Sino Biological provides ready-to-use expression ORF clones without subcloning. The expression ORF clones can be use for transfecting immediately. Now thousands of molecules have been expression validated. Sino Biological has always been committed to providing customers with high quality products and services.
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Human, mouse, rat, monkey, canine, ferret and virus... | |
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• Untagged • C-terminal:Myc, HA, FLAG, His, GFPSpark® and OFPSpark® • N-terminal:Myc, HA, FLAG, His, GFPSpark® and OFPSpark® |
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Full length sequence confirmed, quality assurance |
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Expression validated, high expression level |
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Designed for mammalian expression, stable or transient |
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Enhanced CMV promoter |
The plasmid was transfected into mammalian cells with Sinofection reagent (Cat#STF02) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. EGFP protein expression levels were significantly increased in different cell lines.
• Sino Biological provides ready-to-use expression ORF clones without subcloning. The expression ORF clones can be use for transfecting immediately. Now thousands of molecules have been expression validated.
• The Myc, HA, FLAG, His tagged ORF clones were expression validated by IF (Immunofluorescence). The plasmids were transfected into mammalian cells. After 48h, cells were fixed and blocked, incubated with primary antibodies, then stained with secondary antibody. Finally the fluorescent signals can be detected by fluorescence microscope.
• The GFPSpark® & OFPSpark® tagged ORF clones were transfected into cells transiently. After 48h, the fluorescent signals can be detected by fluorescence microscopy.
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Human PTPN1, N-Flag (HG10304-NF) |
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Human IDH1, N-GFPSpark® (HG12055-ANG) |
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Human SIRT6, C-GFPSpark® (HG12156-ACG) |
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H7N9-NP, C-Flag (VG40110-CF) |
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Human PKM2, C-OFPSpark® (HG11430-ACR) |
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Human MTOR, C-OFPSpark® (HG11508-ACR) |
Catalog No. | Molecule | Product Description | Availability |
HG10304-NF | PTPN1 | Human PTP1B / PTPN1 Gene ORF cDNA clone expression plasmid, N-Flag tag | In stock |
HG11430-ACR | PKM | Human PKM2 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag | In stock |
HG11508-ACR | MTOR | Human MTOR Gene ORF cDNA clone expression plasmid, C-OFPSpark tag | In stock |
HG12054-ACG | RELA | Human RELA / Transcription factor p65 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag | In stock |
HG12055-ANG | IDH1 | Human IDH1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tag | In stock |
HG12125-NF | FTO | Human Fat mass and obesity associated/FTO Gene ORF cDNA clone expression plasmid, N-Flag tag | In stock |
HG12156-ACG | SIRT6 | Human SIRT6 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag | In stock |
HG12283-CF | CD47 | Human CD47 Gene ORF cDNA clone expression plasmid, C-Flag tag | In stock |
MG50843-CF | CXCR5 | Mouse CXCR5 Gene ORF cDNA clone expression plasmid, C-Flag tag | In stock |
RG80100-NF | EGFR | Rat EGFR/HER1 Gene ORF cDNA clone expression plasmid, N-Flag tag | In stock |
VG40042-CF | RSV-F | Human respiratory syncytial virus (RSV) (subtype A, strain A2) Fusion glycoprotein / RSV-F ORF mammalian expression plasmid (Codon Optimized), C-Flag tag | In stock |
VG40110-CF | H7N9-NP | Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag | In stock |
Sino Biological provides a collection of organelle marker plasmids which encoding organelle-specific or structure-specific proteins. The proteins are fused with GFPSpark® or OFPSpark® tag and allow direct visualization of the organelles or structures without manipulation by fluorescence microscopy. Organelle marker plasmids represent a part of expression ORF clone. Learn more about...
The GFPSpark® & OFPSpark® tag ORF clones were transfected into Hela cells transiently. After 48h, the fluorescent signals can be detected by laser scanning confocal microscope (Nikon) (GFPSpark® excitation/ emission max = 487 / 508 nm, OFPSpark® excitation/emission maxima =549 / 566 nm).
Microtuble |
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Human TUBB (HG11626-ACG) |
Mitochondria |
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Human TOMM20 (HG15094-ACG) |
Golgi apparatus |
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Human GOLM1 (HG13066-ACG) |
Autophagosome |
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Human LC3B (HG14555-ACG) |
Cytoskeleton |
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Human EB3 (HG14614-ACR) |
Mitochondria |
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Human PRDX5 (HG11263-ACR) |
Endosome |
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Human RAB5A (HG14013-ANR) |
Endoplasmic Reticulum |
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Human HSPA5 (HG12063-ACR) |