Examples of good or bad IF/ICC Results

For the density of cells used in immunofluorescence staining requires a proper density, different cells are often required to be not exactly the same. According to previous experimental experience, give you some advice:
1. General, the use of the density of adherent cells to control the growth of 60%-80%;
2. The appropriate cell density can ensure the good cell state, get the right results;
3. The appropriate cell density can ensure that the effect of staining, both to avoid false negative results, but also can get beautiful pictures.

Different Cells
 
A431
 
Hela
 
A549
 
HepG2
 
HESc
 
MCF7
 
PC3
 
SHSY5Y
 
SKBR3
 
Raw264.7
 
Mouse Splenocytes
 
Different Express location
 
Cell membrane
 
Cytoplasm
 
Nucleus
 
Lysosome
 
Golgi
 
Mitochondrion
 
Microtubule
 
A431-Cell membrane
A431-Cytoplasm
A431-Nucleus
Hela-Cell membrane
Hela-Cytoplasm
Hela-Nucleus
Hela-Lysosome
Hela-Golgi
Hela-Mitochondrion
Hela-Microtubule
A549-Cytoplasm
A549-Nucleus
A549-Lysosome
HepG2-Cytoplasm
HepG2-Lysosome
HESc-Cytoplasm
HESc-Nucleus
MCF7-Cell membrane
MCF7-Cytoplasm
MCF7-Nucleus
PC3-Cell membrane
SHSY5Y-Cytoplasm
SHSY5Y-Nucleus
SKBR3-Cell membrane
SKBR3-Cytoplasm
Raw264.7-Cell membrane
Mouse Splenocytes-Cell membrane
 
cell membrane
 
chondriosome
 
tubulin
 

Cytoplasm

Golgi
 
Cell neuclus
 
Endoplasmic
 
Losome