To evaluate the extent of the ELISA kit results close to actual values.
A high or low recovery results in false positive or negative result affecting the accuracy of the measured value.
Make sure ELISA kit can detect natural samples and determine its applicable types of samples.
If the types of natural sample are not confirmed, a false positive or negative result may be obtained.
To evaluate the specificity of the ELISA kit and determine the binding ability of other molecules to the paired antibodies.
High cross-reactivity stands for low specificity.
To determine the effects of other molecules to the interaction of antigens with paired antibodies.
High interference stands for low accuracy of the measured value.
To ensure the stability of the ELISA kit for long-term storage.
Better stability of the ELISA kit means longer storage time.
To guarantee if similar results can be obtained when the same samples are repeatedly measured.
Poor precision of the kit will result in poor reproducibility when the same sample is repeatedly measured.
To determine the sensitivity of the ELISA kit.
Lower limit of detection stands for higher sensitivity.
To assess the accuracy of the ELISA kit.
High or low results of linear test will produce false positive or negative data.
IL 4 target | ELISA Kit from Sino Biological Inc. | A high-end brand | A middle-end brand | A low-end brand |
Standard curve | 10.9-700pg/ml | 31.2 - 2,000 pg/mL | 10.24-400pg/mL | 3.12-200ng/ml |
The limit of detection | 2.5pg/ml | 10 pg/mL | 2pg/mL | 0.78ng/ml |
Precision | CV is less than 15% in intra-assay or inter-assay | CV is less than 15% in intra-assay or inter-assay | CV is less than 15% in intra-assay or inter-assay | -- |
Recovery | 80-120% | 80-120% | 80-120% | -- |
Linearity test | 80-120% | 80-120% | 80-120% | -- |
Natural sample test | cell culture supernatant | cell culture supernatant | -- | -- |
Cross reactivity | 37 targets tested | 32 targets tested | 7 targets tested | -- |
Interference test | 37 targets tested | 32 targets tested | 7 targets tested | -- |
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Th9 cell | Th2 cell | T-reg cell | Th22 cell | Th17 cell | Th1 cell | Tfh cell |
IL-10 (KIT10947A) | IL-4 (KIT11846) IL-10 (KIT10947A) |
IL-10 (KIT10947A) TGF β (KIT10804) |
CCL17 (KIT10233) TNF α (KIT10602) |
IL-17A (KIT12047) GM-CSF (KIT10015) TNF α (KIT10602) |
TNF α (KIT10602) | IL-10 (KIT10947A) IL-12 (KITCT011) IL-4 (KIT11846) |
Th9 cell | Th2 cell | T-reg cell | Th22 cell |
IL-10 (KIT10947A) | IL-4 (KIT11846) IL-10 (KIT10947A) |
IL-10 (KIT10947A) TGF β (KIT10804) |
CCL17 (KIT10233) TNF α (KIT10602) |
Th17 cell | Th1 cell | Tfh cell | |
IL-17A (KIT12047) GM-CSF (KIT10015) TNF α (KIT10602) |
TNF α (KIT10602) | IL-10 (KIT10947A) IL-12 (KITCT011) IL-4 (KIT11846) |
Target | Linearity range(pg/ml) | Natural sample test | Cat No. |
Human CXCL9 / MIG | 15.63-1000 | Human serum | KIT10888 |
Human Fractalkine / CX3CL1 | 7.82-500 | Human milk | KIT10636 |
Human GM-CSF/CSF2 | 7.81-500 | Cell culture supernates | KIT10015 |
Human IL10/Interleukin-10 | 18.75-1200 | Cell culture supernates | KIT10947 |
Human IL-12 (IL12A & IL12B Heterodimer) | 39.07-2500 | Cell culture supernates | KITCT011 |
Human IL13/ALRH | 4.69-300 | Cell culture supernates | KIT10369 |
Human IL17A/IL17 | 4.6-300 | Cell culture supernates | KIT12047 |
Human IL32 | 62.5-4000 | Human serum | KIT11064 |
Human IL4 / Interleukin-4 | 7.8-500 | Cell culture supernates | KIT11846 |
Human MCP-1/CCL2 | 3.91-250 | Human serum | KIT10134 |
Human TGFB1 (LAP) / TGF-beta 1 | 46.9-3000 | Human serum | KIT10804 |
Human TNF-alpha/TNFA | 23.44-1500 | Cell culture supernates | KIT10602 |
Human TNF-beta | 7.82-500 | Cell culture supernates | KIT10270 |
Mouse IL16 | 5.17-350 | Mouse serum | KIT51303 |
Mouse IL17A/IL-17A/IL17 ELISA | 23.4-1500 | Cell culture supernates | KIT51065 |
Mouse IL-18 | 23.44-1500 | Mouse serum | KIT50073 |
Mouse IL1A / IL-1A / IL-1F1 | 6.56-420 | Mouse serum | KIT50114 |
Influenza H7N9 (A/Anhui/1/2013) HA | 15.6-1000 | Recombinant protein | KIT40103 |
Influenza H7N9 HA | 15.6-1000 | Recombinant protein | KIT40104 |
Influenza H1N1 HA | 18.75-1200 | Recombinant protein | KIT11055 |
Influenza A H1N1 (A/Puerto Rico/8/1934) HA | 46.88-3000 | Recombinant protein | KIT11684 |
Influenza H3N2 Hemagglutinin / HA | 625-40000 | Recombinant protein | KIT11056 |
Influenza A H4N6 (A/mallard/Ohio/657/2002) HA | 62.5-4000 | Recombinant protein | KIT11714 |
Human Immunodeficiency Virus type 1 (HIV-1) p24 / Capsid Protein p24 | 15.63-1000 | Recombinant protein | KIT11695 |
Human Immunodeficiency Virus type 1 (HIV-1) gp120 | 62.5-4000 | Recombinant protein | KIT11233 |
Human respiratory syncytial virus (RSV) (A2) Fusion glycoprotein | 93.75-6000 | Recombinant protein | KIT11049 |