The ELISA method is a benchmark for quantitation of pathological antigens and there are indeed many variations to this method. ELISAs are adaptable to high-throughput screening because results are rapid, consistent and relatively easy to analyze. The best results have been obtained with the sandwich format, utilizing highly purified, prematched capture and detector antibodies. The resulting signal provides data which is very sensitive and highly specific.
Usually a 96 well polystyrene microplate. These have an 8 × 12 well format and can be used with a wide variety of specialized equipment designed for rapid manipulation of samples including multichannel pipets.