Difference between Immunocytochemistry (ICC) and Immunofluorescence (IF)

There are three concepts which include immunohistochemistry, immunocytochemistry and immunofluorescence. The assay of immunohistochemistry is relatively easy to confirm and distinguish from other two assays. However, what are the assay of immunocytochemistry and immunofluorescence? How to different the assay of immunocytochemistry and immunofluorescence? When we write the research article to be published in magazine, which one word should be exactly word to use?

What is immunocytochemistry / ICC ? what is immunofluorescence / IF? We always ask the questions and don't know how to use the two words. So, many researchers want to the differences between immunocytochemistry / ICC analysis and immunofluorescence / IF analysis.

Let's make clear a few concepts. "Immuno" refers to the staining based on antibodies. "Cyto" always refers to cells, immunocytochemistry is performed on sample of intact cells.Immunofluorescence indicates that a fluorescent tag was used to visualize the marker of interest but fluorescent markers can be used for immunocytochemistry (cells) or for immunohistochemsitry (tissues).

From the scope of the concept, the range of coverage of immunofluorescence its larger and immunofluorescence include immunohistochemistry and immunocytochemistry. Let us put it another way,immunohistochemistry and immunocytochemistry are one type of immunofluorescence.

Immunofluorescence is an assay for confirming the expression and expression location of proteins in nature tissue section. Immunofluorescence can be used on cultured cell lines, tissue sections, or individual cells. Immunocytochemistry is performed on sample of intact cells.Immunofluorescence may be used to analyze the distribution of proteins, glycans, and small biological and non-biological molecules in cells or tissues.

Indirect Assay

Direct Assay