Confirmed by Immunohistochemistry (IHC)

Sino Biological Inc. hase commit to developing a variety of antibodies, including Immunohistochemistry / IHC antibody, Flow Cytometry / FACS antibody, Immunofluorescence / IF / ICC antibody, Immunoprecipitation / IP antibody, Western Blot / WB antibody and ELISA antibody.

There are a variety of detection methods for antibody, and the method can verify each other. The Immunofluorescence / IF / ICC antibodies can be verified by the method of IHC, FAC(FC) and WB. If one result of IHC,FAC(FC) and WB was correct, Immunofluorescence / IF / ICC antibodies were validated that the the Immunofluorescence / IF / ICC antibody is specific to target.

IF Antibodies Confirmed by Immunohistochemistry / IHC

EGFR Catalog: 10001-R043

Immunofluorescence staining of Human CD146 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD146 monoclonal antibody (FITC-conjugated, 10 µg/ml) at 4℃ overnight, and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Immunochemical staining of human EGFR in human uterine cervix cancer with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to uterine gland.

Cynomolgus p53 Catalog: 90001-R002

Immunofluorescence staining of Cynomolgus p53 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Cynomolgus p53 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Immunochemical staining of cynomolgus p53 in human gastric cancer with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

PRDX6 Catalog: 11257-MM01

Confocal immunofluorescence analysis of Human PRDX6 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human PRDX6 monoclonal antibody (15 µg/ml). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody, countstained with Alexa Fluor® 546-conjugated phallotoxins (red) and DAPI (blue). Positive staining was localized to cytoplasm.
Immunochemical staining of human PRDX6 in human brain with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to neuroglia cells.

PLK4 Catalog: 100996-T42

Immunofluorescence staining of PLK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PLK4 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Immunochemical staining of human PLK4 in human stomach with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).