CoV 3CLpro / 3C-like protease Proteins, cDNA Clones Research Reagents

The SARS-CoV-2 genome shares high sequence identity with SARS-CoV. Both viruses critically rely on the activity of viral proteases: the main protease (Mpro, also known as 3CLpro or non-structural protein 5 (nsp5)) and the papain-like protease (PLpro, the protease domain of nsp3) to generate a functional replicase complex and enable viral spread. SCoV-PLpro cleaves ubiquitin and ISG15, known regulators of host innate immune pathways, and inhibition of SCoV-PLpro has been shown to block SARS-CoV replication.

CoV 3CLpro / 3C-like protease Protein (3)

    CoV 3CLpro / 3C-like protease cDNA Clone (32)

    SARS-CoV, isolate WH20

    In expression vector

    CoV 3CLpro / 3C-like protease Lysate (2)

      CoV 3CLpro / 3C-like protease Background

      3C-like protease (3CLpro) is the main protease of Humann Coronavirus. 3C-like protease (3CLpro) is a key enzyme, as it cleaves several sites to produce non-structural proteins that are essential for genome replication and Coronavirus virion production, such as an RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab). SARS-CoV 3CLpro exists as a homodimer and each protomer has an active site.

      CoV 3CLpro / 3C-like protease References

      • Tomonari Muramatsu,et al.Autoprocessing mechanism of severe acute respiratory syndrome coronavirus 3C-like protease (SARS-CoV 3CLpro) from its polyproteins.FEBS Journal.2013
      • Ziebuhr J,Molecular biology of severe acute respiratory syndrome coronavirus. Curr Opin Microbiol.2004
      • Yang H, et al. The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor. Proc Natl Acad Sci USA.2003

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