Anti-TXNDC5 Antibody

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Anti-TXNDC5 Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-TXNDC5 Antibody
Validated applications
WB,IHC-P,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human TXNDC5
Immunogen
E. coli-derived Human TXNDC5 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human TXNDC5 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-TXNDC5 Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:5000-1:20000
IHC-P 1:1000-1:10000
ICC-IF 1:100-1:500
IP IP:0.5-1μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-TXNDC5 Antibody (Rabbit Polyclonal antibody) Images

Anti-TXNDC5 rabbit polyclonal antibody at 1:5000 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: HeLa Whole Cell Lysate

Lane C: HEK293 Whole Cell Lysate

Lane D: 293T Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:48 kDa

Observed band size:50 kDa

Immunochemical staining of human TXNDC5 in human tonsil with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human TXNDC5 in human stomach with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunofluorescence staining of TXNDC5 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TXNDC5 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm.

TXNDC5 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

4 µL anti-TXNDC5 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-TXNDC5 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 48 kDa

Observed band size :50 kDa

Anti-TXNDC5 Antibody: Synonyms

Anti-TXNDC5 Antibody

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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