Anti-IGFBP2 Antibody, Rabbit Polyclonal

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Anti-IGFBP2 Antibody, Rabbit Polyclonal General Information

Product name
Anti-IGFBP2 Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Rhesus
Specificity
Rhesus IGFBP2
Immunogen
Recombinant Rhesus IGFBP-2 / IGFBP2 protein (Catalog#90083-C08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rhesus IGFBP-2 / IGFBP2 (Catalog#90083-C08H; XP_001087071.1; Met1-Gln324). IGFBP-2 / IGFBP2 specific IgG was purified by Rhesus IGFBP-2 / IGFBP2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IGFBP2 Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:100-1:500
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IGFBP2 Antibody, Rabbit Polyclonal Images

Immunochemical staining of Rhesus IGFBP2 in Rhesus kidney with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).
Immunochemical staining of Rhesus IGFBP2 in Rhesus heart with rabbit polyclonal antibody (1:300, formalin-fixed paraffin embedded sections).

cyno IGFBP2 was immunoprecipitated using:

Lane A:0.5 mg SH-SY5Y Whole Cell Lysate

2 µL anti-cyno IGFBP2 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-cyno IGFBP2 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 35 kDa

Observed band size: 35 kDa

Anti-IGFBP rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: A549 Whole Cell Lysate

Lane C: SH-SY5Y Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:35 kDa

Observed band size:35 kDa

IGFBP2 Background Information

IGFBP-2, also known as IGFBP2, is a insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs , control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified. The six IGFBPs are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. It inhibits IGF-mediated growth and developmental rates.
Full Name
insulin-like growth factor binding protein 2, 36kDa
References
  • Han VK, et al. (1996) The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab. 81(7): 2680-93.
  • Binkert C, et al. (1989) Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGFBP-2) . EMBO J. 8(9):2497-502.
  • Wolf E, et al. (2000) Effects of IGFBP-2 overexpression in vitro and in vivo. Pediatr Nephrol. 14 (7):572-8.

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