Anti-PARP Antibody, Rabbit Polyclonal

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Anti-PARP Antibody, Rabbit Polyclonal General Information

Product name
Anti-PARP Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human PARP
Immunogen
E. coli-derived Human PARP / PARP1 fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human PARP / PARP1 fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.

Anti-PARP Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ICC-IF 1:100-1:500
IP IP:1-5μL/mg of μLysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PARP Antibody, Rabbit Polyclonal Images

Anti-PARP rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:113 kDa

Observed band size:120 kDa

Immunofluorescence staining of PARP1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PARP1 polyclonal antibody (dilution ratio 1:200) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

PARP1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

4 µL anti-PARP1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-PARP1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 113 kDa

Observed band size :120 kDa

Anti-PARP Antibody, Rabbit Polyclonal: Synonyms

Anti-ADPRT Antibody; Anti-ADPRT1 Antibody; Anti-ARTD1 Antibody; Anti-pADPRT-1 Antibody; Anti-PARP Antibody; Anti-PARP-1 Antibody; Anti-PPOL Antibody

PARP Background Information

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
Full Name
poly (ADP-ribose) polymerase 1
Research Areas
References
  • Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
  • Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
  • Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
  • Ahell I. et al., 2008, Nature. 451: 81-85.

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