Anti-PARP Antibody, Mouse Monoclonal

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Anti-PARP Antibody, Mouse Monoclonal General Information

Product name
Anti-PARP Antibody, Mouse Monoclonal
Validated applications
IHC-P
Species reactivity
Reacts with: Human
Specificity
Human PARP
Immunogen
E. coli-derived Human PARP / PARP1 fragment
Preparation
Produced in mouse immunized with E. coli-derived Human PARP / PARP1 fragment. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #11
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PARP Antibody, Mouse Monoclonal Validated Applications

Application Dilution
IHC-P 1:100-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PARP Antibody, Mouse Monoclonal Images

Immunochemical staining of human PARP in human testis with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Anti-PARP Antibody, Mouse Monoclonal: Synonyms

Anti-ADPRT Antibody; Anti-ADPRT1 Antibody; Anti-ARTD1 Antibody; Anti-pADPRT-1 Antibody; Anti-PARP Antibody; Anti-PARP-1 Antibody; Anti-PPOL Antibody

PARP Background Information

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme that modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrated to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors are thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
Full Name
poly (ADP-ribose) polymerase 1
Research Areas
References
  • Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
  • Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
  • Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
  • Ahell I. et al., 2008, Nature. 451: 81-85.

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Mouse MAb

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