Recombinant Anti-P4HB Antibody, Rabbit Monoclonal General Information
Recombinant Anti-P4HB Antibody, Rabbit Monoclonal
Reacts with: Mouse
Recombinant Mouse P4HB protein (Catalog#50638-M08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse P4HB (rM P4HB; Catalog#50638-M08H; NP_035162.1; Met1-Lys506).
Monoclonal Rabbit IgG Clone #023
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunochemical staining of mouse P4HB in mouse liver with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunochemical staining of mouse P4HB in mouse intestine with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunochemical staining of mouse P4HB in mouse brain with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).
Immunofluorescence staining of P4HB in NIH/3T3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-mouse P4HB monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Protein disulfide-isomerase, also known as Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55 and P4HB, is a peripheral membrane protein that belongs to the protein disulfide isomerase family. P4HB is highly abundant. In some cell types, it seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources. P4HB localizes near CD4-enriched regions on lymphoid cell surfaces. It is identified by mass spectrometry in melanosome fractions from stage I to stage IV. P4HB reduces and may activate fusogenic properties of HIV-1 gp12 surface protein, thereby enabling HIV-1 entry into the cell. P4HB catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. P4HB may therefore cause structural modifications of exofacial proteins. Inside the cell, it seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, P4HB functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, it facilitates aggregation (anti-chaperone activity). P4HB may be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. It also acts as a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
prolyl 4-hydroxylase, beta polypeptide
Kivirikko KI, et al., 1989, FASEB J., 3 (5): 1609-17.
Pihlajaniemi T, et al.,1991, J Hepatol., 13, Suppl 3: S2
Fenouillet E., et al., 2001, J. Infect. Dis. 183:744-752.
Gevaert K., et al., 2003, Nat. Biotechnol. 21:566-569.
Barbouche R., et al., 2003, J. Biol. Chem. 278:3131-3136.
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