Anti-IL36 gamma Antibody, Rabbit Polyclonal

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Anti-IL36 gamma Antibody, Rabbit Polyclonal General Information

Product name
Anti-IL36 gamma Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse IL36 gamma
Immunogen
Recombinant Mouse IL36G protein (Catalog#51179-M07E)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse IL36G (Catalog#51179-M07E; Q8R460; Gly13-Ser164). IL36G specific IgG was purified by Mouse IL36G affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL36 gamma Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 4-6 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL36 gamma Antibody, Rabbit Polyclonal Images

Anti-IL36g rabbit polyclonal antibody at 1:500 dilution

Lane A: NCI-H1299 Whole Cell Lysate

Lane B: HelaS3 Whole Cell Lysate

Lane C: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:19 kDa

Observed band size:22 kDa

(We are unsure as to the identity of these extra bands.)

Mouse IL36g was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

2 µL anti-Mouse IL36g rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse IL36g rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 22 kDa

Observed band size: 22 kDa

Anti-IL36 gamma Antibody, Rabbit Polyclonal: Synonyms

Anti-Il1f9 Antibody; Anti-IL36g Antibody

IL36 gamma Background Information

Full Name
interleukin 36, gamma
Related Pathways
  • IL1 signaling pathway
    IL1 signaling pathway
References
  • Dinarello CA. (2002) The IL-1 family and inflammatory diseases. Clin Exp Rheumatol. 20(5): 1-13.
  • Berglof E, et al. (2003) IL-1Rrp2 expression and IL-1F9 (IL-1H1) actions in brain cells. J Neuroimmunol. 139(1-2): 36-43.
  • Dunn E, et al. (2001) Annotating genes with potential roles in the immune system: six new members of the IL-1 family. Trends Immunol.22(10): 533-6.
  • Towne JE, et al. (2004) Interleukin (IL)-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP to activate the pathway leading to NF-kappaB and MAPKs. J Biol Chem. 279(14): 13677-88.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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