Anti-CD8 alpha Antibody (PerCP) (Rabbit Monoclonal antibody) General Information
Anti-CD8 alpha Antibody (PerCP)
Reacts with: Mouse
Mouse CD8 alpha
Recombinant Mouse CD8a protein (Catalog#50389-M08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD8a (rM CD8a; Catalog#50389-M08H; NP_001074579.1; Met 1-Tyr 196) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
Monoclonal Rabbit IgG Clone #208
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
2 μl/Test, 0.1 mg/ml
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow cytometric analysis of CD8α expression on spleen lymphocytes. BALB/c splenocytes were stained with PerCP-conjugated anti-Mouse CD8α (50389-R208-P). The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.
T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.
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