Anti-FCGR3 rabbit monoclonal antibody at 1:500 dilution
Lane A: C6 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:29 kDa
Observed band size:38 kDa
(We are unsure as to the identity of these extra bands.)
mFCGR3 was immunoprecipitated using:
Lane A:0.5 mg RAW246.7 Whole Cell Lysate
4µL anti-mFCGR3 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Anti-mFCGR3 rabbit monoclonal antibody,at 1:100 dilution
Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size :50 kDa