Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal

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Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD16/FCGR3
Has cross-reactivity in ELISA with
Human CD64
No cross-reactivity in ELISA with
Human CD32a
Human CD32b
Human CD16a
Human CD16b
Cynomolgus FCGR3
Immunogen
Recombinant Mouse CD16 / FCGR3 protein (Catalog#50326-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16 / FCGR3 (rM CD16 / FCGR3; Catalog#50326-M08H; P08508-1; Met 1-Thr 215).
Source
Monoclonal Rabbit IgG Clone #015
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal Images

Anti-FCGR3 rabbit monoclonal antibody at 1:500 dilution

Lane A: C6 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:29 kDa

Observed band size:38 kDa

(We are unsure as to the identity of these extra bands.)

mFCGR3 was immunoprecipitated using:

Lane A:0.5 mg RAW246.7 Whole Cell Lysate

4µL anti-mFCGR3 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-mFCGR3 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 29 kDa

Observed band size :50 kDa

Recombinant Anti-CD16/FCGR3 Antibody, Rabbit Monoclonal: Synonyms

Anti-CD16 Antibody

CD16/FCGR3 Background Information

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Full Name
Fc receptor, IgG, low affinity III
Research Areas
References
  • Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
  • Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
  • Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.

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