Recombinant Anti-CD16/FCGR3 Antibody (APC), Rabbit Monoclonal

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Recombinant Anti-CD16/FCGR3 Antibody (APC), Rabbit Monoclonal General Information

Product name
Recombinant Anti-CD16/FCGR3 Antibody (APC), Rabbit Monoclonal
Validated applications
FCM
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD16/FCGR3
Immunogen
Recombinant Mouse CD16 / FCGR3 protein (Catalog#50326-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD16 / FCGR3 (rM CD16 / FCGR3; Catalog#50326-M08H; P08508-1; Met1-Thr215) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Source
Monoclonal Rabbit IgG Clone #002
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Conjugate
APC
Concentration
10 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Recombinant Anti-CD16/FCGR3 Antibody (APC), Rabbit Monoclonal Images

Flow cytometric analysis of Mouse FCGR3(CD16) expression on mouse splenocytes. BABL/c splenocytes were stained with APC-conjugated anti-Mouse FCGR3(CD16) and FITC-conjugated anti-Mouse CD3 (BD Pharmingen™ Cat. No. 553061). The dot plots were derived from events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-CD16/FCGR3 Antibody (APC), Rabbit Monoclonal: Synonyms

Anti-CD16 Antibody

CD16/FCGR3 Background Information

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Full Name
Fc receptor, IgG, low affinity III
Research Areas
References
  • Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
  • Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
  • Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

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Mouse MAb

Rabbit PAb

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