Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Polyclonal

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Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Polyclonal General Information

Product name
Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse Carbonic Anhydrase 8/CA8
Immunogen
Recombinant Mouse Carbonic Anhydrase VIII / CA8 protein (Catalog#50767-M08E)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse Carbonic Anhydrase VIII / CA8 (Catalog#50767-M08E; P28651; Met1-Gln291). Carbonic Anhydrase VIII / CA8 specific IgG was purified by Mouse Carbonic Anhydrase VIII / CA8 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:5000-1:25000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Polyclonal Images

Immunochemical staining of mouse CA8 in mouse brain with rabbit polyclonal antibody at 1:25000 dilution, formalin-fixed paraffin embedded sections.

Mouse CA8 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

0.5 µL anti-Mouse CA8 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CA8 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 33 kDa

Observed band size: 37 kDa

Anti-CA8 rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:37 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Polyclonal: Synonyms

Anti-AW546993 Antibody; Anti-Ca8 Antibody; Anti-Cals Antibody; Anti-Cals1 Antibody; Anti-Car8 Antibody; Anti-Carp Antibody; Anti-RP23-180H12.1 Antibody; Anti-wdl Antibody

Carbonic Anhydrase 8/CA8 Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Full Name
carbonic anhydrase VIII
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.

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