Recombinant Anti-Interferon Gamma Antibody, Rabbit Monoclonal

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Recombinant Anti-Interferon Gamma Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-Interferon Gamma Antibody, Rabbit Monoclonal
Validated applications
FCM
Species reactivity
Reacts with: Mouse
Specificity
Mouse Interferon Gamma
Immunogen
Recombinant Mouse Interferon Gamma / IFNG Protein (Catalog#50709-MNAH)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Interferon Gamma / IFNG (rM Interferon Gamma / IFNG; Catalog#50709-MNAH; NP_032363.1; Met1-Cys155).
Source
Monoclonal Rabbit IgG Clone #373
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Interferon Gamma Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Interferon Gamma Antibody, Rabbit Monoclonal Images

Flow cytometric analysis of Mouse Interferon gamma expression on BABL/c splenocytes. BABL/c splenocytes were stimulated for 4-6 hours with PMA and Ionomycin in the presence of GolgiPlug. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Mouse Interferon gamma, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Recombinant Anti-Interferon Gamma Antibody, Rabbit Monoclonal: Synonyms

Anti-Ifg Antibody; Anti-IFN-g Antibody

Interferon Gamma Background Information

IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.
Full Name
interferon, gamma
Research Areas
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
References
  • Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
  • Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
  • Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
  • Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
  • Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
  • Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.

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