Anti-Transferrin Antibody, Rabbit Polyclonal

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Anti-Transferrin Antibody, Rabbit Polyclonal General Information

Product name
Anti-Transferrin Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Transferrin
Immunogen
Recombinant Human transferrin protein (Catalog#11019-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Transferrin / TF (rh Transferrin; Catalog#11019-H08H; NP_001054.1; Met 1-Pro 698). Transferrin specific IgG was purified by human Transferrin affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Transferrin Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:1000-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Transferrin Antibody, Rabbit Polyclonal Images

TF was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

2 µL anti-TF rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-TF rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 100 kDa

Observed band size: 100 kDa

Anti-TF rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 whole cell lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:77 kDa

Observed band size:85 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Transferrin Antibody, Rabbit Polyclonal: Synonyms

Anti-HEL-S-71p Antibody; Anti-PRO1557 Antibody; Anti-PRO2086 Antibody; Anti-TFQTL1 Antibody; Anti-Transferrin Antibody

Transferrin Background Information

Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. This glycoprotein is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of Transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte / pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. Transferrins are iron binding transport proteins which bind Fe3+ ion in association with the binding of an anion, usually bicarbonate. This transferrin binds only one Fe3+ ion per protein molecule. Transports iron ions from the hemolymph into the eggs during the vitellogenic stage. Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. When a transferrin loaded with iron encounters with a transferring receptor on cell surface, transferring binds to it and, as a consequence, is transported into the cell in a visicle by receptor-mediated endocytosis. The PH is reduced by hydrogen iron pumps. The lower pH causes transferrin to release its iron ions. The receptor is then transported through the endocytic cycle back to the cell surface, ready for another round of iron uptake. Each transferrin molecule has the ability to carry two iron ions in the ferric form.
Full Name
transferrin
References
  • Ponka P, et al. (1998) Function and regulation of transferrin and ferritin. Semin Hematol. 35(1): 35-54.
  • Wagner E, et al. (1990) Transferrin-polycation conjugates as carriers for DNA uptake into cells. Proc Natl Acad Sci. 87(9): 3410-4.
  • Cheng Y, et al. (2004) Structure of the human transferrin receptor-transferrin complex. Cell. 116 (4): 565-76.
  • Iron-deficiency and estrogen are associated with ischemic stroke by up-regulating transferrin to induce hypercoagulability
    Author
    Tang, X;Fang, M;Cheng, R;Zhang, Z;Wang, Y;Shen, C;
    Year
    2019
    Journal
    bioRxiv
    Application
    WB
  • Transferrin plays a central role to maintain coagulation balance by interacting with clotting factors
    Author
    Tang, X;Zhang, Z;Fang, M;Han, Y;Wang, S;Xue, M;Li, Y;
    Year
    2019
    Journal
    bioRxiv
    Application
    WB
  • Transferrin plays a central role in coagulation balance by interacting with clotting factors
    Author
    Tang, X;Zhang, Z;Fang, M;Han, Y;Wang, G;Wang, S;Xue, M;Li, Y;Zhang, L;Wu, J;Yang, B;Mwangi, J;Lu, Q;Du, X;Lai, R;
    Year
    2019
    Journal
    Cell Res.
    Application
    WB

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