TNF-alpha / TNFA Neutralizing Antibody

RRID Number: AB_2860204
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TNF-alpha / TNFA Neutralizing Antibody General Information

Product name
TNF-alpha / TNFA Neutralizing Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human TNF-alpha
Immunogen
Recombinant Human TNF-alpha / TNFA Protein (Catalog#10602-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TNF-alpha / TNFA (rh TNF-alpha / TNFA; Catalog#10602-HNAE; NP_000585.2; Val77-Leu233). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #MM0N1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

TNF-alpha / TNFA Neutralizing Antibody Validated Applications

Notes
Block: In a functional ELISA which immobilized recombinant Human TNFR1 (Catalog# 10872-H08H) at 1μg/mL (100 µL/well) in the plate, the Mouse Anti-Human TNFα Monoclonal Antibody (Catalog# 10602-MM0N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog# 10602-HNAE-B) to human TNFR1,the EC50 is 0.74 μg/mL;In a functional ELISA which immobilized recombinant Human TNFR2 (Catalog# 10417-H08H) at 2μg/mL (100 µL/well) in the plate, the Mouse Anti-Human TNFα Monoclonal Antibody (Catalog# 10602-MM0N1) can block the binding of 0.2 μg/mL of biotinylated Human TNFα (Catalog# 10602-HNAE-B) to human TNFR1,the EC50 is 0.82 μg/mL.
Neutralization: The neutralization activity of antibody is measured by its ability to neutralize TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 13.5-54 ng/mL in the presence of 2 ng/mL Recombinant Human TNFa and 1 μg/mL actinomycin D.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

TNF-alpha / TNFA Neutralizing Antibody Images

The neutralization activity of antibody is measured by its ability to neutralize TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. The Neutralization titer (IC50) is typically 13.5-54 ng/mL in the presence of 0.25 ng/mL Recombinant Human TNFa and 1 μg/mL actinomycin D.

TNF-alpha / TNFA Neutralizing Antibody: Synonyms

Anti-DIF Antibody; Anti-TNF-alpha Antibody; Anti-TNFA Antibody; Anti-TNFSF2 Antibody

TNF-alpha Background Information

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & Antibody

Full Name
tumor necrosis factor
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • NF-kB (NFkB) Pathway
    NF-kB (NFkB) Pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
  • Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
  • Lupus serum IgG induces microglia activation through Fc fragment dependent way and modulated by B-cell activating factor
    Author
    Yang, C;Hou, X;Feng, Q;Li, Y;Wang, X;Qin, L;Yang, P;
    Year
    2019
    Journal
    J Transl Med

Standard Antibody Development Service

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Mouse MAb

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