Anti-SerpinD1 Antibody, Rabbit Polyclonal

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Anti-SerpinD1 Antibody, Rabbit Polyclonal General Information

Product name
Anti-SerpinD1 Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human SerpinD1
Immunogen
Recombinant Human SerpinD1 protein (Catalog#10295-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human SerpinD1 (rh SerpinD1; Catalog#10295-H08H; NP_000176.2; Met 1-Ser 499). SerpinD1 specific IgG was purified by human SerpinD1 affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-SerpinD1 Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:1000-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-SerpinD1 Antibody, Rabbit Polyclonal Images

SerpinD1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-SerpinD1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SerpinD1 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 57 kDa

Observed band size: 70 kDa

Anti-SerpinD1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:57 kDa

Observed band size:70 kDa

Anti-SerpinD1 Antibody, Rabbit Polyclonal: Synonyms

Anti-D22S673 Antibody; Anti-HC2 Antibody; Anti-HCF2 Antibody; Anti-HCII Antibody; Anti-HLS2 Antibody; Anti-LS2 Antibody; Anti-SerpinD1 Antibody; Anti-THPH10 Antibody

SerpinD1 Background Information

SerpinD1, also known as heparin cofactor II, is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.
Full Name
serpin peptidase inhibitor, clade D (heparin cofactor), member 1
References
  • Rau JC, et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study. Exp Mol Pathol. 87(3): 178-83.
  • Aihara K, et al. (2009) Heparin cofactor II as a novel vascular protective factor against atherosclerosis. J Atheroscler Thromb. 16(5): 523-31.

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