Anti-PIK3CA Antibody, Rabbit Polyclonal

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Anti-PIK3CA Antibody, Rabbit Polyclonal General Information

Product name
Anti-PIK3CA Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF
Species reactivity
Reacts with: Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Specificity
Human PIK3CA
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human PIK3CA
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PIK3CA, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PIK3CA Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ICC-IF 1:1500-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PIK3CA Antibody, Rabbit Polyclonal Images

Immunofluorescence staining of PIK3CA in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PIK3CA polyclonal antibody (1:5000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Anti-PIK3CA rabbit polyclonal antibody at 1:500 dilution.

Lane A: Mouse heart tissue lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:124 kDa

Observed band size:90 kDa

(We are unsure as to the identity of these extra bands.)

Anti-PIK3CA Antibody, Rabbit Polyclonal: Synonyms

Anti-CLAPO Antibody; Anti-CLOVE Antibody; Anti-CWS5 Antibody; Anti-MCAP Antibody; Anti-MCM Antibody; Anti-MCMTC Antibody; Anti-p110-alpha Antibody; Anti-PI3K Antibody; Anti-PI3K-alpha Antibody

PIK3CA Background Information

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 11 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.
Full Name
phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Research Areas
Related Pathways
  • Actin Dynamics Signaling Pathway
    Actin Dynamics Signaling Pathway
  • AKT Signaling Pathway
    AKT Signaling Pathway
  • AMPK Signaling Pathway
    AMPK Signaling Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • G Protein-coupled Receptors Signaling
    G Protein-coupled Receptors Signaling
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • MAPK-Erk Pathway
    MAPK-Erk Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
References
  • Limaye N, Kangas J, Mendola A, et al. Somatic Activating PIK3CA Mutations Cause Venous Malformation. American Journal of Human Genetics. 2015;97(6):914-921.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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