Anti-PEDF Antibody, Rabbit Polyclonal

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Anti-PEDF Antibody, Rabbit Polyclonal General Information

Product name
Anti-PEDF Antibody, Rabbit Polyclonal
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human PEDF
Immunogen
Recombinant Human SerpinF1 protein (Catalog#11104-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human SerpinF1 (rh SerpinF1; Catalog#11104-H08H; NP_002606.3; Met 1-Pro 418). SerpinF1 specific IgG was purified by human SerpinF1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PEDF Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:5000-1:10000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PEDF Antibody, Rabbit Polyclonal Images

SerpinF1 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-SerpinF1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SerpinF1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 46 kDa

Observed band size: 46 kDa

Anti-SerpinF1 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:46 kDa

Observed band size:46 kDa

(We are unsure as to the identity of these extra bands.)

Anti-PEDF Antibody, Rabbit Polyclonal: Synonyms

Anti-EPC-1 Antibody; Anti-OI12 Antibody; Anti-OI6 Antibody; Anti-PEDF Antibody; Anti-PIG35 Antibody

PEDF Background Information

Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein that has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.
Full Name
serpin peptidase inhibitor, clade F,member 1
References
  • Ren, JG. et al., 2005, Med Hypotheses. 64 (1): 74-8.
  • Filleur, S. et al., 2009. J Cell Biochem. 106 (5): 769-75.
  • Kawaguchi, T. et al., 2010, Curr Mol Med. 10 (3): 302-11.
  • Yamagishi, SI. et al., 2010, Curr Mol Med. 10 (3): 284-91.
  • Nakamura, T. et al., 2010, Curr Mol Med. 10 (3): 312-6.

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