PD-L1 / B7-H1 / CD274 Blocking Antibody

RRID Number: AB_2860080
EliteRmab®, manufactured by recombinant technology
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PD-L1 / B7-H1 / CD274 Blocking Antibody General Information

Product name
PD-L1 / B7-H1 / CD274 Blocking Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human PD-L1
Has cross-reactivity in ELISA with
Cynomolgus PD-L1 (Catalog # 90251-C08H)
Human PD-L1 (Catalog # 10084-H08H)
No cross-reactivity in ELISA with
Human PD-L2 (Catalog # 10292-H08H)
Immunogen
Recombinant Human PD-L1 / B7-H1 / CD274 protein (Catalog#10084-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human PD-L1 / B7-H1 / CD274 (rh PD-L1 / B7-H1 / CD274; Catalog#10084-H08H; NP_054862.1; Met1-Thr239) and was produced using recombinant antibody technology.
Source
Monoclonal Rabbit IgG Clone #R639
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

PD-L1 / B7-H1 / CD274 Blocking Antibody Validated Applications

Notes
Block: In a functional ELISA which immobilized recombinant Human PD1 /Fc Chimera (Catalog # 10377-H02H) at 10μg/mL (100 µL/well) in the plate, the Rabbit Anti-Human PD-L1 Monoclonal Antibody (Catalog # 10084-R639) can block the binding of 0.1 μg/mL of recombinant Human PD-L1 (Catalog # 10084-H02H) to human PD1, the EC50 is 0.83 μg/mL;
Please Note: Optimal concentrations/dilutions should be determined by the end user.

PD-L1 / B7-H1 / CD274 Blocking Antibody: Synonyms

Anti-B7-H Antibody; Anti-B7-H1 Antibody; Anti-B7H1 Antibody; Anti-PD-L1 Antibody; Anti-PDCD1L1 Antibody; Anti-PDCD1LG1 Antibody; Anti-PDL1 Antibody

PD-L1 Background Information

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
Full Name
CD274 molecule
Research Areas
References
  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.
  • Phage display-identified PD-L1-binding peptides reinvigorate T-cell activity and inhibit tumor progression
    Author
    Gurung, S;Khan, F;Gunassekaran, G;Yoo, J;Poongkavithai Vadevoo, S;Permpoon, U;Kim, S;Kim, H;Kim, I;Han, H;Park, J;Kim, S;Lee, B;
    Year
    2020
    Journal
    Biomaterials
    Application
    FACS

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