Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal

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Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal
Validated applications
ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human N Cadherin
Immunogen
Recombinant Human N-Cadherin / CD325 / CDH2 Protein (Catalog#11039-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human N-Cadherin / CD325 / CDH2 (rh N-Cadherin / CD325 / CDH2; Catalog#11039-H08H; NP_001783.2; Met 1-Ala 724).
Source
Monoclonal Rabbit IgG Clone #001
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
ELISA 1:5000-1:10000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal Images

Flow cytometry analysis of N-Cadherin on HeLa cells. HeLa cells were harvested with (Left) or without (Right) trypsinization [please note, the epitope is sensitive to trypsin] and stained with either purified Rabbit IgG isotype control (dashed line) or Rabbit Anti-Human CD325 antibody [solid line, and Clone R001 (upper), Clone R020 (bottom)] at matching concentrations, then a FITC-conjugated second step antibody.

Recombinant Anti-N Cadherin Antibody, Rabbit Monoclonal: Synonyms

Anti-CD325 Antibody; Anti-CDHN Antibody; Anti-CDw325 Antibody; Anti-NCAD Antibody

N Cadherin Background Information

Cadherins are calcium-dependent cell adhesion proteins, and they preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 2 (CDH2), also known as N-Cadherin (neuronal) (NCAD), is a single-pass transmembrane protein and a cadherin containing 5 cadherin domains. N-Cadherin displays a ubiquitous expression pattern but with different expression levels between endocrine cell types. CDH2 (NCAD) has been shown to play an essential role in normal neuronal development, which is implicated in an array of processes including neuronal differentiation and migration, and axon growth and fasciculation. In addition, N-Cadherin expression was upregulated in human HSC during activation in culture, and function or expression blocking of N-Cadherin promoted apoptosis. During apoptosis, N-Cadherin was cleaved into 20-100 kDa fragments. It may provide a novel target for therapies that are directed toward intimal proliferative disorders, including restenosis and vascular bypass graft failure. N-Cadherin is associated with tumor aggressiveness and metastatic potential and may contribute to tumor progression.
Full Name
cadherin 2, type 1, N-cadherin (neuronal)
Related Pathways
  • Canonical Wnt Pathway
    Canonical Wnt Pathway
References
  • Jones M, et al. (2002) N-cadherin upregulation and function in response of smooth muscle cells to arterial injury. Arterioscler Thromb Vasc Biol. 22(12): 1972-7.
  • Nagi C, et al. (2005) N-cadherin expression in breast cancer: correlation with an aggressive histologic variant--invasive micropapillary carcinoma. Breast Cancer Res Treat. 94(3): 225-35.
  • Schrick C, et al. (2007) N-cadherin regulates cytoskeletally associated IQGAP1/ERK signaling and memory formation. Neuron. 55(5): 786-98.
  • Li K, et al. (2010) Downregulation of N-cadherin expression inhibits invasiveness, arrests cell cycle and induces cell apoptosis in esophageal squamous cell carcinoma. Cancer Invest. 28(5): 479-86.

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