Anti-MMP-2 Antibody, Mouse Monoclonal

RRID Number: AB_2860073
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Anti-MMP-2 Antibody, Mouse Monoclonal General Information

Product name
Anti-MMP-2 Antibody, Mouse Monoclonal
Validated applications
WB,ELISA,FCM
Species reactivity
Reacts with: Human
Specificity
Human MMP-2
No cross-reactivity in ELISA with
Human MMP1
Human MMP7
Human MMP9
Human cell lysate (293 cell line)
Immunogen
Recombinant Human MMP-2 protein (Catalog#10082-HNAH)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MMP-2 (rh MMP-2; Catalog#10082-HNAH; NP_004521.1; Met 1-Cys660). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2a Clone #05
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-MMP-2 Antibody, Mouse Monoclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:5000-1:10000
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-MMP-2 Antibody, Mouse Monoclonal Images

Anti-MMP2 mouse monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:74 kDa

Observed band size:55 kDa

Flow cytometric analysis of Human MMP2 expression on MG63 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human MMP2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-MMP-2 Antibody, Mouse Monoclonal: Synonyms

Anti-CLG4 Antibody; Anti-CLG4A Antibody; Anti-MMP-2 Antibody; Anti-MMP-II Antibody; Anti-MONA Antibody; Anti-TBE-1 Antibody

MMP-2 Background Information

Matrix Metalloproteinase-2 (MMP-2) is an enzyme that degrades components of the extracellular matrix and thus plays a pivotal role in cell migration during physiological and pathological processes. MMP-2 expression is dependent on extracellular matrix metalloproteinase inducer (EMMPRIN), Her2/neu, growth factors, cytokines, and hormones. Pro-MMP-2 activation needs MT1-MMP and TIMP-2 contribution. MMP-2 is changed in distribution and increased in amount in the ventral cochlear nucleus after unilateral cochlear ablation. A low level of MMP-2 is linked to a favorable prognosis in patients with a hormone receptor-negative tumor, usually associated with high risk. As a zymogen requiring proteolytic activation for catalytic activity, MMP-2 has been implicated broadly in the invasion and metastasis of many cancer model systems, including human breast cancer (HBC). Blocking MMP-2 secretion and activation during breast carcinoma development may decrease metastasis. The detection of active MMP-2 alone or the rate of pro-MMP-2 and active MMP-2 is considered a very sensitive indicator of cancer metastasis. Modulation of MMP-2 expression and activation through specific inhibitors and activators may thus provide a new mechanism for breast cancer treatment.
Full Name
matrix metallopeptidase 2
Research Areas
References
  • Thompson EW, et al. (1994) Collagen induced MMP-2 activation in human breast cancer. Breast Cancer Res Treat. 31(2-3): 357-70.
  • Jezierska A, et al. (2009) Matrix metalloproteinase-2 involvement in breast cancer progression: a mini-review. Med Sci Monit. 15(2): RA32-40.
  • Fredrich M, et al. (2010) MMP-2 is involved in synaptic remodeling after cochlear lesion. Neuroreport. 21(5): 324-7.

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Mouse MAb

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