IL17 / IL17a Neutralizing Antibody

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IL17 / IL17a Neutralizing Antibody General Information

Product name
IL17 / IL17a Neutralizing Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human IL17
Has cross-reactivity in ELISA with
Canine IL17A (Catalog # 70102-DNAE)
No cross-reactivity in ELISA with
Mouse IL17A (Catalog # 51065-MNAE)
Immunogen
Recombinant Human IL17 / IL17a protein (Catalog#12047-HNAE)
Preparation
This antibody was obtained from a mouse immunized with purified, recombinant Human IL17 / IL17a (rHuman IL17 / IL17a; Catalog#12047-HNAE; Q16552; Ile20-Ala155) and was produced using recombinant antibody technology.
Source
Monoclonal Mouse IgG1 Clone #M237
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

IL17 / IL17a Neutralizing Antibody Validated Applications

Notes
Block: In a functional ELISA which immobilized recombinant Human IL17a (Catalog# 12047-HNAE) at 1μg/mL (100 µL/well) in the plate, the Rabbit anti-Human IL17a Monoclonal Antibody (Catalog# 12047-M237) can block the binding of 0.5μg/mL of biotinylated recombinant Human IL17Ra (Catalog# 10895-H03H-B) to human IL17a,the EC50 is 0.60 μg/mL.
Neutralization: The neutralization activity of IL17a Neutralizing Antibody is measured by its ability to neutralize IL6 secretion induced by human IL17a (12047-HNAE) in the HFF human foreskin fibroblast. The Neutralization Titer (IC50) is typically 0.23-0.94 µg/mL in the presence of 50 ng/mL Recombinant Human IL17a (Catalog # 12047-HNAE) .
Please Note: Optimal concentrations/dilutions should be determined by the end user.

IL17 / IL17a Neutralizing Antibody Images

The ability of Human IL-17a Neutralizing Mouse mAb to inhibit IL-17A-induced IL-6 production was analyzed. IL6 secretion in the HFF human foreskin fibroblast induced by 50 ng/mL of Recombinant Human IL17a (12047-HNAE) is neutralized by increasing concentrations of mouse anti-Human IL17a neutralizing Monoclonal Antibody (Catalog # 12047-M237), as measured by IL6 Quantitative ELISA Kit (Catalog # KIT10395).The IC50 is typically 0.23-0.94 μg/mL.

IL17 / IL17a Neutralizing Antibody: Synonyms

Anti-CTLA-8 Antibody; Anti-CTLA8 Antibody; Anti-IL-17 Antibody; Anti-IL-17A Antibody; Anti-IL17 Antibody

IL17 Background Information

IL17, also known as IL17a, is a cytokine that belongs to the IL-17 family. Cytokines are proteinaceous signaling compounds that are major mediators of the immune response. They control many different cellular functions including proliferation, differentiation, and cell survival/apoptosis but are also involved in several pathophysiological processes including viral infections and autoimmune diseases. Cytokines are synthesized under various stimuli by a variety of cells of both the innate (monocytes, macrophages, dendritic cells) and adaptive (T- and B-cells) immune systems. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis, and multiple sclerosis.
Full Name
interleukin 17A
Research Areas
Related Pathways
  • IL12 signaling pathway
    IL12 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
References
  • Andoh A, et al. (2002) IL-17 selectively down-regulates TNF-alpha-induced RANTES gene expression in human colonic subepithelial myofibroblasts. J Immunol. 169(4):1683-7.
  • Kotake S, et al. (1999) IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis. J Clin Invest. 103(9):1345-52.
  • Laan M, et al. (1999) Neutrophil recruitment by human IL-17 via C-X-C chemokine release in the airways. J Immunol. 162(4):2347-52.
  • Shin HC, et al. (1999) Regulation of IL-17, IFN-gamma and IL-10 in human CD8(+) T cells by cyclic AMP-dependent signal transduction pathway. Cytokine. 10(11):841-50.
  • Th17 Lymphocytes Induce Neuronal Cell Death in a Human iPSC-Based Model of Parkinson's Disease
    Author
    Sommer, A;Maxreiter, F;Krach, F;Fadler, T;Grosch, J;Maroni, M;Graef, D;Eberhardt, E;Riemenschneider, MJ;Yeo, GW;Kohl, Z;Xiang, W;Gage, FH;Winkler, J;Prots, I;Winner, B;
    Year
    2018
    Journal
    Cell Stem Cell
    Application
    cell-based

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