IL-6R / CD126 Neutralizing Antibody

RRID Number: AB_2860170
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IL-6R / CD126 Neutralizing Antibody General Information

Product name
IL-6R / CD126 Neutralizing Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human IL-6R
No cross-reactivity in ELISA with
Rat IL6R (Catalog # 81072-R02H)
Mouse IL6R (Catalog # 50280-M08H)
Canine IL6R (Catalog # 70117-D08H)
Immunogen
Recombinant Human IL-6R / CD126 protein (Catalog#10398-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-6R / CD126 (rh IL-6R / CD126; Catalog#10398-H08H; NP_000556.1; Met1-Pro365). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #3H7D3H1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Endotoxin
Please contact us for more information.
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

IL-6R / CD126 Neutralizing Antibody Validated Applications

Notes
Block: This IL6R Neutralizing Antibody can not block the binding of recombinant IL6 (Catalog # 10395-HNAE) to its recombinant receptor IL6 Rα (Catalog # 10398-H08H) .
Neutralization: The neutralization activity of IL6R Neutralizing Antibody is measured by its ability to neutralize IL6 Rα-mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Titer (IC50) is typically 0.15-0.6 µg/mL in the presence of 50 ng/mL Recombinant Human IL6 Rα (Catalog # 10398-H08H) and 100 ng/mL Recombinant Human IL6 (Catalog # 10395-HNAE) .
Please Note: Optimal concentrations/dilutions should be determined by the end user.

IL-6R / CD126 Neutralizing Antibody Images

IL-6 Rα Enhancement of IL6-dependent Inhibition of Cell Proliferation was Neutralized by Human IL-6 Rα Neutralizing Antibody in the M1 mouse myeloid leukemia cell line. Enhancement of Recombinant Human IL6 (100 ng/mL) activity elicited by Recombinant Human IL-6 Rα (50 ng/mL) is neutralized by increasing concentrations of Human IL6 Rα neutralizing Monoclonal Antibody (Catalog # 10398-MM08H). The IC50 is typically 0.15-0.6 μg/mL.

IL-6R / CD126 Neutralizing Antibody: Synonyms

Anti-CD126 Antibody; Anti-gp80 Antibody; Anti-IL-6R Antibody; Anti-IL-6R-1 Antibody; Anti-IL-6RA Antibody; Anti-IL6Q Antibody; Anti-IL6RA Antibody; Anti-IL6RQ Antibody

IL-6R Background Information

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp13. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp13 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.
Full Name
interleukin 6 receptor
Research Areas
Related Pathways
  • AKT Signaling Pathway
    AKT Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
  • Autophagy Pathway
    Autophagy Pathway
References
  • Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
  • Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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