Anti-IL-4 Antibody, Mouse Monoclonal

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Anti-IL-4 Antibody, Mouse Monoclonal General Information

Product name
Anti-IL-4 Antibody, Mouse Monoclonal
Validated applications
ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human IL-4
Immunogen
Recombinant Human IL4/IL-4/Interleukin-4 Protein (Catalog#11846-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL4/IL-4/Interleukin-4 (rh IL4/IL-4/Interleukin-4; Catalog#11846-HNAE; P05112-1; His25-Ser153). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Clone #04
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-IL-4 Antibody, Mouse Monoclonal Validated Applications

Notes
ELISA(Det): This antibody will detect Human IL4 / IL-4 / Interleukin-4 in ELISA pair set (Catalog: # SEK11846). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11846-MM05).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-IL-4 Antibody, Mouse Monoclonal: Synonyms

Anti-BCGF-1 Antibody; Anti-BCGF1 Antibody; Anti-BSF-1 Antibody; Anti-BSF1 Antibody; Anti-IL-4 Antibody; Anti-Interleukin-4 Antibody

IL-4 Background Information

Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.
Full Name
interleukin 4
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
References
  • Grunewald SM. et al., 1998, J Immunol. 160 (8): 4004-9.
  • Susanne M. et al, 1997, THE JOURNAL OF BIOLOGICAL CHEMISTRY. 272 (3): 1480-3.
  • Nishikubo K. et al., 2003, Gene Ther. 10 (26): 2119-25.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

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Mouse MAb

Rabbit PAb

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