Anti-HSP60 Antibody, Rabbit Polyclonal

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Anti-HSP60 Antibody, Rabbit Polyclonal General Information

Product name
Anti-HSP60 Antibody, Rabbit Polyclonal
Validated applications
WB,ICC/IF,IP
Species reactivity
Reacts with: Human
Specificity
Human HSP60
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human HSPD1 / HSP60
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HSPD1 / HSP60, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-HSP60 Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ICC-IF 1:300-1:10000
IP 0.5-2 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-HSP60 Antibody, Rabbit Polyclonal Images

Immunofluorescence staining of HSPD1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human HSPD1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Mitochondrion matrix.

HSPD1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg K562 Whole Cell Lysate

2 µL anti-HSPD1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-HSPD1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 60 kDa

Observed band size: 60 kDa

Anti-HSPD1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 whole cell lysate

Lane B: K562 whole cell lysate

Lane C: NIH-3T3 whole cell lysate

Lane D: A431 whole cell lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:60 kDa

Observed band size:60 kDa

(We are unsure as to the identity of these extra bands.)

Anti-HSP60 Antibody, Rabbit Polyclonal: Synonyms

Anti-CPN60 Antibody; Anti-GROEL Antibody; Anti-HLD4 Antibody; Anti-HSP-60 Antibody; Anti-HSP60 Antibody; Anti-HSP65 Antibody; Anti-HuCHA60 Antibody; Anti-SPG13 Antibody

HSP60 Background Information

HSPD1, also known as HSP6, is a member of the chaperonin family. HSPD1 may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. It may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. HSPD1 gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13). Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4); also called mitochondrial HSP6 chaperonopathy or MitCHAP-6 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. HSPD1 is cinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.
Full Name
heat shock 60kDa protein 1 (chaperonin)
References
  • Hansen J J, et al. (2002) Hereditary spastic paraplegia SPG13 is associated with a mutation in the gene encoding the mitochondrial chaperonin Hsp60. Am J Hum Genet. 70: 1328-32.
  • Magen D, et al. (2008) Mitochondrial Hsp60 chaperonopathy causes an autosomal-recessive neurodegenerative disorder linked to brain hypomyelination and leukodystrophy. Am J Hum Genet. 83: 30-42.
  • Venner TJ, et al. (1990) Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families. DNA Cell Biol. 9 (8): 545-52.

Standard Antibody Development Service

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Mouse MAb

Rabbit PAb

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