Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal

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Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal General Information

Product name
Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal
Validated applications
WB,ELISA,ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human Hsp27/HSPB1
No cross-reactivity in ELISA with
E.coli cell lysate
Immunogen
Recombinant Human HSP27 Protein (Catalog#10351-H08E)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1 – Lys 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #4E 8H3
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:1000-1:2000
IP 4-6 μL/mg of lysate
ELISA(Det) 1:1000-1:10000
Notes
ELISA(Det): This antibody will detect Human HSPB1 / HSP 27 in ELISA pair set (Catalog: # SEK10351). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10351-R017).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal Images

Anti-HSP27 mouse monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:26 kDa

HSP27 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-HSP27 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HSP27 mouse monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal: Synonyms

Anti-CMT2F Antibody; Anti-HEL-S-102 Antibody; Anti-HMN2B Antibody; Anti-HS.76067 Antibody; Anti-Hsp25 Antibody; Anti-HSP27 Antibody; Anti-HSP28 Antibody; Anti-SRP27 Antibody

Hsp27/HSPB1 Background Information

Full Name
heat shock 27kDa protein 1
Research Areas
Related Pathways
  • VEGF Signaling Pathway
    VEGF Signaling Pathway
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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