Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal


Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal General Information

Product name
Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal
Validated applications
Species reactivity
Reacts with: Human
Human Hsp27/HSPB1
No cross-reactivity in ELISA with
E.coli cell lysate
Recombinant Human HSP27 Protein (Catalog#10351-H08E)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met 1 – Lys 205). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #4E 8H3
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal Validated Applications

Application Dilution
WB 1:500-1:2000
ELISA 1:1000-1:2000
IP 4-6 μL/mg of lysate
ELISA(Det) 1:1000-1:10000
ELISA(Det): This antibody will detect Human HSPB1 / HSP 27 in ELISA pair set (Catalog: # SEK10351). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10351-R017).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal Images

Anti-HSP27 mouse monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:26 kDa

HSP27 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

2 µL anti-HSP27 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-HSP27 mouse monoclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 25 kDa

Observed band size: 25 kDa

Anti-Hsp27/HSPB1 Antibody, Mouse Monoclonal: Synonyms

Anti-CMT2F Antibody; Anti-HEL-S-102 Antibody; Anti-HMN2B Antibody; Anti-HS.76067 Antibody; Anti-Hsp25 Antibody; Anti-HSP27 Antibody; Anti-HSP28 Antibody; Anti-SRP27 Antibody

Hsp27/HSPB1 Background Information

Full Name
heat shock 27kDa protein 1
Research Areas
Related Pathways
  • VEGF Signaling Pathway
    VEGF Signaling Pathway
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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