Recombinant Anti-Factor IX Antibody, Rabbit Monoclonal

RRID Number: AB_2860350
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-Factor IX Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-Factor IX Antibody, Rabbit Monoclonal
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Factor IX
Immunogen
Recombinant Human Coagulation Factor IX / FIX / F9 protein (Catalog#11503-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Coagulation Factor IX / FIX / F9 (rh Coagulation Factor IX / FIX / F9; Catalog#11503-H08H; AAB59620.1; Met 1-Thr 461).
Source
Monoclonal Rabbit IgG Clone #034
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Factor IX Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
IP 0.1-0.5 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Factor IX Antibody, Rabbit Monoclonal Images

Anti-F9 rabbit monoclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lane B: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:55 kDa

F9 was immunoprecipitated using:

Lane A:0.5 mg A431 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

0.5 µL anti-F9 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.

Primary antibody:

Anti-F9 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 55 kDa

Observed band size :55 kDa

Recombinant Anti-Factor IX Antibody, Rabbit Monoclonal: Synonyms

Anti-Coagulation factor 9 Antibody; Anti-Coagulation factor IX Antibody; Anti-FIX Antibody; Anti-HEMB Antibody; Anti-P19 Antibody; Anti-PTC Antibody; Anti-THPH8 Antibody

Factor IX Background Information

Coagulation factor IX, also known as Christmas factor, Plasma thromboplastin component and PTC, is a secreted protein which belongs to the peptidase S1 family. Coagulation factor IX / F9 contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one?peptidase S1 domain. Coagulation factor IX / F9 is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca2+ons, phospholipids, and factor VIIIa. Defects in Coagulation factor IX / F9 are the cause of thrombophilia due to factor IX defect which is a hemostatic disorder characterized by a tendency to thrombosis. Defects in Coagulation factor IX / F9 are also the cause of recessive X-linked hemophilia B ( HEMB ) which also known as Christmas disease.
Full Name
coagulation factor IX
References
  • Onay U.V., et al., 2003, Br. J. Haematol. 120:656-659.
  • Vidal F., et al., 2000, Br. J. Haematol. 111:549-551.
  • Simioni P., et al., 2009, N. Engl. J. Med. 361:1671-1675.
  • Espinos C., et al., 2009, Haematologica 88:235-236.

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