Anti-F9 rabbit monoclonal antibody at 1:500 dilution
Lane A: A431 Whole Cell Lysate
Lane B: Jurkat Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:52 kDa
Observed band size:55 kDa
F9 was immunoprecipitated using:
Lane A:0.5 mg A431 Whole Cell Lysate
Lane B:0.5 mg Jurkat Whole Cell Lysate
0.5 µL anti-F9 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Anti-F9 rabbit monoclonal antibody,at 1:500 dilution
Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size :55 kDa