EGF / Epidermal Growth Factor Neutralizing Antibody

EliteRmab®, manufactured by recombinant technology
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EGF / Epidermal Growth Factor Neutralizing Antibody General Information

Product name
EGF / Epidermal Growth Factor Neutralizing Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human EGF
Has cross-reactivity in ELISA with
Canine EGF (Catalog # 170013-D07E)
Mouse EGF (Catalog # 50482-MNCH)
Immunogen
Recombinant Human EGF / Epidermal Growth Factor Protein (Catalog#10605-HNAE)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human EGF / Epidermal Growth Factor (rh EGF / Epidermal Growth Factor; Catalog#10605-HNAE; NP_001954.2; Asn971-Arg1023) and was produced using recombinant antibody technology.
Source
Monoclonal Rabbit IgG Clone #R001
Purification
Protein A
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 3 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

EGF / Epidermal Growth Factor Neutralizing Antibody Validated Applications

Notes
Block: In a functional ELISA, 1.2 µg/mL of this antibody will block >80% of the binding of 5 µg/mL of biotinylated Human EGF (Catalog# 10605-H01H-B) to immobilized Recombinant Human EGFR (Catalog# 10001-H08B) coated at 10 µg/mL (100 µL/well) in the plate.
Neutralization: The neutralization activity of EGF Neutralizing Antibody is Measured by its ability to neutralize EGF induced proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. The Neutralization titer (IC50) is typically 2.5-7.5 μg/mL in the presence of 2 ng/mL Recombinant Human EGF.
Please Note: Optimal concentrations/dilutions should be determined by the end user.

EGF / Epidermal Growth Factor Neutralizing Antibody Images

Cell Proliferation Induced by EGF was Neutralized by Human EGF Antibody. Recombinant Human EGF stimulates proliferation in the Balb/c 3T3 mouse embryonic fibroblast cell line. Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized by increasing concentrations of Human EGF Monoclonal Antibody (Catalog # 10605-R001). The IC50 is typically 2.5-7.5 μg/mL.

EGF / Epidermal Growth Factor Neutralizing Antibody: Synonyms

Anti-Epidermal Growth Factor Antibody; Anti-HOMG4 Antibody; Anti-URG Antibody

EGF Background Information

EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.
Full Name
epidermal growth factor
Related Pathways
  • EGFR Signaling Pathway
    EGFR Signaling Pathway
  • Jak-Stat Signaling Pathway
    Jak-Stat Signaling Pathway
References
  • Chen JX, et al. (2011) Involvement of c-Src/STAT3 signal in EGF-induced proliferation of rat spermatogonial stem cells. Mol Cell Biochem. 358(1-2):67-73.
  • Guo Y, et al. (2012) Correlations among ERCC1, XPB, UBE2I, EGF, TAL2 and ILF3 revealed by gene signatures of histological subtypes of patients with epithelial ovarian cancer. Oncol Rep. 27(1):286-92.
  • Kim S, et al. (2012) Smad7 acts as a negative regulator of the epidermal growth factor (EGF) signaling pathway in breast cancer cells. Cancer Lett. 314(2):147-54.
  • Chatterton RT Jr, et al. (2010) Breast ductal lavage for assessment of breast cancer biomarkers. Horm Cancer. 1(4):197-204.
  • Modulation of hepatitis B virus infection by epidermal growth factor secreted from liver sinusoidal endothelial cells
    Author
    Chen, SW;Himeno, M;Koui, Y;Sugiyama, M;Nishitsuji, H;Mizokami, M;Shimotohno, K;Miyajima, A;Kido, T;
    Year
    2020
    Journal
    Sci Rep
    Application
    binding

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