CLU was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
2 µL anti-CLU rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein A/G.
Anti-CLU rabbit polyclonal antibody,at 1:100 dilution
Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size :55 kDa