Anti-Clusterin Antibody, Mouse Monoclonal

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Anti-Clusterin Antibody, Mouse Monoclonal General Information

Product name
Anti-Clusterin Antibody, Mouse Monoclonal
Validated applications
WB,ELISA,FCM,ICC/IF
Species reactivity
Reacts with: Human
Specificity
Human Clusterin
No cross-reactivity in ELISA with
Mouse CLU / Clusterin / Apolipoprotein J / Apo-J
Human cell lysate (293 cell line)
Immunogen
Recombinant Human Clusterin / Apolipoprotein J / Apo-J Protein (Catalog#11297-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Clusterin / Apolipoprotein J / Apo-J (rh Clusterin / Apolipoprotein J / Apo-J; Catalog#11297-H08H; NP_001822.2; Met 1-Glu 501). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #9E2E2
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Clusterin Antibody, Mouse Monoclonal Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:5000-1:10000
ICC-IF 1:100-1:500
FCM 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Clusterin Antibody, Mouse Monoclonal Images

Immunofluorescence staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CLU monoclonal antibody (1:300) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human CLU expression on A549 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Anti-CLU mouse monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:57 kDa

Anti-Clusterin Antibody, Mouse Monoclonal: Synonyms

Anti-AAG4 Antibody; Anti-APO-J Antibody; Anti-APOJ Antibody; Anti-CLI Antibody; Anti-CLU1 Antibody; Anti-CLU2 Antibody; Anti-KUB1 Antibody; Anti-NA1/NA2 Antibody; Anti-SGP-2 Antibody; Anti-SGP2 Antibody; Anti-SP-40 Antibody; Anti-TRPM-2 Antibody; Anti-TRPM2 Antibody

Clusterin Background Information

Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.
Full Name
clusterin
References
  • Silkensen JR, et al. (1994) The role of clusterin in tissue injury. Biochem Cell Biol. 72(11-12): 483-8.
  • Naik RR, et al. (2002) Biomimetic synthesis and patterning of silver nanoparticles. Nat Mater. 1(3): 169-72.
  • Djeu JY, et al. (2009) Clusterin and chemoresistance. Adv Cancer Res. 105: 77-92.

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