Anti-CD8 alpha Antibody

1/1
Price:
Size:
Number:

Anti-CD8 alpha Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-CD8 alpha Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD8 alpha
Immunogen
Recombinant Human CD8a / Lyt2 Protein (Catalog#10980-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD8a / Lyt2 (rh CD8a / Lyt2; Catalog#10980-H08H; NP_001759.3; Met1-Asp182). CD8a / Lyt2 specific IgG was purified by Human CD8a / Lyt2 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD8 alpha Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution
WB 1:500-1:1000
ELISA 1:25000-1:50000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD8 alpha Antibody (Rabbit Polyclonal antibody) Images

Anti-CD8A rabbit polyclonal antibody at 1:500 dilution.

Lane A: U87MG Membrane Lysate

Lane B: K562 Membrane Lysate

Lane C: Hela Membrane Lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:32 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CD8 alpha Antibody: Synonyms

Anti-CD8 Antibody; Anti-CD8 alpha Antibody; Anti-Leu-2 Antibody; Anti-Leu2 Antibody; Anti-MAL Antibody; Anti-p32 Antibody; Anti-p32 186910 Antibody

CD8 alpha Background Information

T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
Full Name
CD8a molecule
References
    References Devine, L. et al., 2000, J Immunol. 164 (2): 833-8. Arcaro, A. et al., 2000, J Immunol. 165 (4): 2068-76. Saha, K. et al., 2001, Nat Med. 7 (1): 65-72. Romero, P. et al., 2005, Eur J Immunol. 35 (11): 3092-4.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

Add to Cart Successfully Add to Cart Failed Shopping cart is being updated, please wait U.S.A.