Recombinant Anti-CD32B/Fcgr2b Antibody, Rabbit Monoclonal

RRID Number: AB_2860125
EliteRmab®, manufactured by recombinant technology
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Recombinant Anti-CD32B/Fcgr2b Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-CD32B/Fcgr2b Antibody, Rabbit Monoclonal
Validated applications
WB,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human CD32B/Fcgr2b
Has cross-reactivity in ELISA with
Human CD64
Human CD16b
No cross-reactivity in ELISA with
Human CD32a
Human CD16a
Mouse FCGR2B
Immunogen
Recombinant Human CD32b protein (Catalog#10259-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD32b extracellular domain (rh CD32b; Catalog#10259-H08H; Ala 46-Pro 217; NP_001002274.1).
Source
Monoclonal Rabbit IgG Clone #110
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-CD32B/Fcgr2b Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
WB 1:500-1:1000
IHC-P 1:2500-1:15000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-CD32B/Fcgr2b Antibody, Rabbit Monoclonal Images

Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of B cells and some other mononuclear cell (1:10000).

Anti-CD32b rabbit monoclonal antibody at 1:500 dilution

Lane A: Daudi Whole Cell Lysate

Lane B: THP1 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:38 kDa

Recombinant Anti-CD32B/Fcgr2b Antibody, Rabbit Monoclonal: Synonyms

Anti-CD32 Antibody; Anti-CD32A Antibody; Anti-CD32B Antibody; Anti-CDw32 Antibody; Anti-Fc gamma RII Antibody; Anti-Fc gamma RIIB Antibody; Anti-FCG2 Antibody; Anti-FcGR Antibody; Anti-FCGR2 Antibody; Anti-FCGR21 Antibody; Anti-FCGR2A Antibody; Anti-IGFR2 Antibody

CD32B/Fcgr2b Background Information

Full Name
Fc fragment of IgG, low affinity IIb, receptor (CD32)
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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