Recombinant Anti-CD19 Antibody, Rabbit Monoclonal

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Recombinant Anti-CD19 Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-CD19 Antibody, Rabbit Monoclonal
Validated applications
ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human CD19
No cross-reactivity in ELISA with
Mouse CD19
Immunogen
Recombinant Human CD19 protein (Catalog#11880-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; P15391-1; Met1-Lys291).
Source
Monoclonal Rabbit IgG Clone #410
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-CD19 Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-CD19 Antibody, Rabbit Monoclonal Images

CD19 was immunoprecipitated using:

Lane A:0.5 mg Daudi Whole Cell Lysate

2 µL anti-CD19 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD19 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 61 kDa

Observed band size: 100 kDa

Recombinant Anti-CD19 Antibody, Rabbit Monoclonal: Synonyms

Anti-B4 Antibody; Anti-CVID3 Antibody

CD19 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in the threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell co-receptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.
Full Name
CD19 molecule
Research Areas
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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