Anti-CD16/FCGR3 Antibody (PE), Mouse Monoclonal

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Anti-CD16/FCGR3 Antibody (PE), Mouse Monoclonal General Information

Product name
Anti-CD16/FCGR3 Antibody (PE), Mouse Monoclonal
Validated applications
FCM
Species reactivity
Reacts with: Human
Specificity
Human CD16/FCGR3
Immunogen
Recombinant Human CD16a/Fc gamma RIIIa Protein (Catalog#10389-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD16a/Fc gamma RIIIa (rh CD16a/Fc gamma RIIIa; Catalog#10389-H08H; P08637-1; Met1-Gln208,176F) and conjugated with PE under optimum conditions, the unreacted PE was removed.
Source
Monoclonal Mouse IgG1 Clone #23
Purification
Protein A
Formulation
Aqueous solution containing 0.5% BSA and 0.03%ProClin300
Conjugate
PE
Concentration
5 μl/Test, 0.1 mg/ml
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze !

Anti-CD16/FCGR3 Antibody (PE), Mouse Monoclonal Images

Flow cytometric analysis of Human CD16 expression on human whole blood lymphocytes. Cells were stained with PE-conjugated anti-Human CD16. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Anti-CD16/FCGR3 Antibody (PE), Mouse Monoclonal: Synonyms

Anti-CD16 Antibody; Anti-Fc gamma RIII Antibody; Anti-FCRIII Antibody

CD16/FCGR3 Background Information

Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.
Full Name
Fc receptor, IgG, low affinity III
Research Areas
References
  • Edberg JC, et al. (2002) Genetic linkage and association of Fcgamma receptor IIIA (CD16A) on chromosome 1q23 with human systemic lupus erythematosus. Arthritis Rheum. 46(8): 2132-40.
  • Li P, et al. (2002) Recombinant CD16A-Ig forms a homodimer and cross-blocks the ligand binding functions of neutrophil and monocyte Fcgamma receptors. Mol Immunol. 38(7): 527-38.
  • Li P, et al. (2007) Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG ligands. J Biol Chem. 282(9): 6210-21.

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