Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal

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Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human Carbonic Anhydrase 8/CA8
Immunogen
Recombinant Human Carbonic Anhydrase VIII / CA8 protein (Catalog#10469-H08E)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase VIII / CA8 (rh Carbonic Anhydrase VIII / CA8; Catalog#10469-H08E; NP_004047.3; Met1-Gln290).
Source
Monoclonal Rabbit IgG Clone #008
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
WB 1:500-1:1000
IP 0.2-1 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal Images

Anti-CA8 rabbit monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:38 kDa

CA8 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg A549 Whole Cell Lysate

0.5 µL anti-CA8 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CA8 rabbit monoclonal antibody,at 1:150 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 37 kDa

Observed band size: 37 kDa

Recombinant Anti-Carbonic Anhydrase 8/CA8 Antibody, Rabbit Monoclonal: Synonyms

Anti-CA-VIII Antibody; Anti-CALS Antibody; Anti-CAMRQ3 Antibody; Anti-CARP Antibody; Anti-MGC120502 Antibody; Anti-MGC99509 Antibody

Carbonic Anhydrase 8/CA8 Background Information

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
Full Name
carbonic anhydrase VIII
References
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.

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