Anti-Apolipoprotein E Antibody, Rabbit Polyclonal

RRID Number: AB_2848192
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Anti-Apolipoprotein E Antibody, Rabbit Polyclonal General Information

Product name
Anti-Apolipoprotein E Antibody, Rabbit Polyclonal
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human Apolipoprotein E
Immunogen
Recombinant Human APOE / apolipoprotein E protein (Catalog#10817-H30E)
Preparation
Produced in rabbits immunized with purified, recombinant Human APOE / apolipoprotein E (rh APOE / apolipoprotein E; Catalog#10817-H30E; P02649; Lys 19-His 317). APOE / apolipoprotein E specific IgG was purified by Human APOE / apolipoprotein E affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Apolipoprotein E Antibody, Rabbit Polyclonal Validated Applications

Application Dilution
ELISA 1:5000-1:10000
IHC-P 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Apolipoprotein E Antibody, Rabbit Polyclonal Images

Immunochemical staining of human APOE in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

Anti-Apolipoprotein E Antibody, Rabbit Polyclonal: Synonyms

Anti-AD2 Antibody; Anti-APO-E Antibody; Anti-ApoE4 Antibody; Anti-LDLCQ5 Antibody; Anti-LPG Antibody

Apolipoprotein E Background Information

Apolipoprotein E (ApoE) is a 34.2 kDa glycosylated protein with 299 amino acid residues. There are three isoforms in human (apoE2, apoE3, and apoE4) due to different amino acid residues at positions 112 and 158. ApoE is synthesized predominantly in the liver, but also by cells in the spleen, brain, lung, kidney, ovary, adrenal, and muscle tissues. Hepatic parenchyma cells are the main apoE producing cells in mammalian body, probably accounting for two thirds to three fourths of the plasma apoE . In the nervous system, apoE mRNA is present in neurons, astrocytes, ependymal cells, nonmyelinating Schwann cells, but not in microglia, oligodendroglia, choroidal cells, or myelinating Schwann cells. ApoE produced by mammalian cells exists in different forms, monomers, dimers, modified, unmodified, lipid-rich, and lipid-poor, and so forth. ApoE plays a double-role in immune responses. Both apoE containing lipoproteins and multimers of synthetic apoE peptides inhibited proliferation of cultured lymphocytes by inhibiting DNA synthesis and reducing phospholipid turnover in T cells. ApoE can also affect innate and acquired immune responses in vitro by its ability to suppress stimulation of cultured neutrophils. ApoE can bind lipopolysaccharide (LPS), attenuate the inflammatory response, and thus reduce LPS induced lethality. Injection of LPS stimulated higher expression of inflammatory cytokines like interleukin (IL)-1β, IL-12, and interferon-γ (IFN-γ), as well as IL-6.
Full Name
apolipoprotein E
References
  • Mahley RW. (1988) Apolipoprotein E: cholesterol transport protein with expanding role in cell biology. Science. 240(4852): 622-30.
  • Aleshkov S, et al. (1989) Interaction of nascent apoe2, apoe3, and apoe4 isoforms expressed in mammalian cells with amyloid peptide. Relevance to Alzheimer's disease. Biochemistry. 36(34): 10571-80.
  • Hussain MM, et al. (1997) Synthesis, modification, and flotation properties of rat hepatocyte apolipoproteins. Biochimica et Biophysica Acta. 101(1): 90-101.
  • GP73 is upregulated by hepatitis C virus (HCV) infection and enhances HCV secretion
    Author
    Hu, L;Yao, W;Wang, F;Rong, X;Peng, T;
    Year
    2014
    Journal
    PLoS ONE
    Application
    IF
  • Hepatocyte nuclear factor 4α and downstream secreted phospholipase A2 GXIIB regulate production of infectious hepatitis C virus
    Author
    Li, X;Jiang, H;Qu, L;Yao, W;Cai, H;Chen, L;Peng, T;
    Year
    2014
    Journal
    J. Virol.
  • Long-chain fatty acyl-coenzyme A suppresses hepatitis C virus infection by targeting virion-bound lipoproteins
    Author
    Li, X;Li, J;Feng, Y;Cai, H;Li, YP;Peng, T;
    Year
    2020
    Journal
    Antiviral Res.
    Application
    WB;IFA
  • Apolipoprotein M, identified as a novel hepatitis C virus (HCV) particle associated protein, contributes to HCV assembly and interacts with E2 protein
    Author
    Cai, H;Yao, W;Huang, J;Xiao, J;Chen, W;Hu, L;Mai, R;Liang, M;Chen, D;Jiang, N;Zhou, L;Peng, T;
    Year
    2020
    Journal
    Antiviral Res.

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