Recombinant Anti-ALK-1 Antibody, Rabbit Monoclonal

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Recombinant Anti-ALK-1 Antibody, Rabbit Monoclonal General Information

Product name
Recombinant Anti-ALK-1 Antibody, Rabbit Monoclonal
Validated applications
WB,IP
Species reactivity
Reacts with: Human
Specificity
Human ALK-1
Immunogen
Recombinant Human ALK-1 / ACVRL1 protein (Catalog#10066-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ALK-1 / ACVRL1 (rh ALK-1 / ACVRL1; Catalog#10066-H08H; NP_000011.2; Met1-Gln118).
Source
Monoclonal Rabbit IgG Clone #124
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Recombinant Anti-ALK-1 Antibody, Rabbit Monoclonal Validated Applications

Application Dilution
WB 1:500-1:2000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Recombinant Anti-ALK-1 Antibody, Rabbit Monoclonal Images

ALK1 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

Lane B:0.5 mg K562 Whole Cell Lysate

2 µL anti-ALK1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ALK1 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 56 kDa

Observed band size: 56 kDa

Anti-ALK1 rabbit monoclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lane B: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:56 kDa

Observed band size:41 kDa

Recombinant Anti-ALK-1 Antibody, Rabbit Monoclonal: Synonyms

Anti-ACVRLK1 Antibody; Anti-ALK-1 Antibody; Anti-ALK1 Antibody; Anti-HHT Antibody; Anti-HHT2 Antibody; Anti-ORW2 Antibody; Anti-SKR3 Antibody; Anti-TSR-I Antibody

ALK-1 Background Information

Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.
Full Name
activin A receptor type II-like 1
References
  • French Rendu-Osler network,et al. (2004) Molecular screening of ALK1/ACVRL1 and ENG genes in hereditary hemorrhagic telangiectasia in France. Hum Mutat. 23(4): 289-299.
  • Simon M, et al. (2006) Association of a polymorphism of the ACVRL1 gene with sporadic arteriovenous malformations of the central nervous system. J Neurosurg. 104(6): 945-9.
  • Argyriou L, et al. (2006) Novel mutations in the ENG and ACVRL1 genes causing hereditary hemorrhagic teleangiectasia. Int J Mol Med. 17(4):655-9.

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