Influenza A H2N2 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified


Influenza A H2N2 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified General Information

Product name
Influenza A H2N2 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified
Validated applications
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
H2N2 Hemagglutinin/HA
Recombinant H2N2 (A/Canada/720/2005) HA / Hemagglutinin protein (Catalog#11688-V08H)
Produced in rabbits immunized with purified, recombinant H2N2 (A/Canada/720/2005) HA / Hemagglutinin (Catalog#11688-V08H; AAY28987.1; Met 1-Gln 516). H2N2 (A/Canada/720/2005) HA / Hemagglutinin specific IgG was purified by H2N2 (A/Canada/720/2005) HA / Hemagglutinin affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H2N2 Hemagglutinin / HA Antibody, Rabbit PAb, Antigen Affinity Purified Validated Applications

Application Dilution
WB his antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect H2N2 (A/Canada/720/2005) HA in WB. Using a DAB detection system, the detection limit for H2N2 (A/Canada/720/2005) HA is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
ELISA 1:5000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin/HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
  • H2 influenza A virus is not pathogenic in Tmprss2 knock-out mice
    Lambertz, RLO;Gerhauser, I;Nehlmeier, I;Gärtner, S;Winkler, M;Leist, SR;Kollmus, H;Pöhlmann, S;Schughart, K;
    Virol. J.

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