Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb

RRID Number: AB_2860372

Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb General Information

Product name
Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb
Validated applications
Application notes
IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)
H1N1 Hemagglutinin/HA
No cross-reactivity in ELISA with
H1N1 (A/BrevigMission/1/1918) HA
H1N1 (A/Brisbane/02/2018) HA
H1N1 (A/Brisbane/59/2007) HA
H1N1 (A/Brisbane/59/2007) HA1
H1N1 (A/California/04/2009) HA
H1N1 (A/California/04/2009) HA1
H1N1 (A/California/07/2009) HA
H1N1 (A/Guangdong-Maonan/SWL1536/2019) / (A/Hawaii/70/2019) HA
H1N1 (A/Michigan/45/2015) HA
H1N1 (A/New Caledonia/20/1999) HA
H1N1 (A/Ohio/UR06-0091/2007) HA
H1N1 (A/Solomon Islands/3/2006)
H1N1 (A/Wisconsin/588/2019) / (A/Victoria/2570/2019) HA
H1N1 (A/WSN/1933) HA
H1N2 (A/swine/Guangxi/13/2006) HA
H1N3 (A/duck/NZL/160/1976)
H3N2 (A/Brisbane/10/2007) HA
H3N2 (A/Cambodia/e0826360/2020) HA
H3N2 (A/Hong Kong/2671/2019) HA
H3N2 (A/Hong Kong/45/2019) HA
H3N2 (A/Hong Kong/4801/2014) HA
H3N2 (A/Kansas/14/2017) HA
H3N2 (A/Singapore/INFIMH-16-0019/2016) HA
H3N2 (A/Switzerland/9715293/2013) HA
H5N1 (A/Anhui/1/2005) HA
H5N1 (A/Anhui/1/2005) HA2
Influenza B (B/Brisbane/60/2008) HA
Influenza B (B/Colorado/06/2017) HA
Influenza B (B/Florida/4/2006) HA
Influenza B (B/PHUKET/3073/2013) HA
Influenza B (B/Washington/02/2019) HA
Recombinant H1N1 (A/Puerto Rico/8/1934) HA protein (Catalog#11684-V08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant H1N1 (A/Puerto Rico/8/1934) HA (Catalog#11684-V08H; ABD77675.1; Met 1-Gln 528). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #2F1A7
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Influenza A H1N1 Hemagglutinin / HA Antibody, Mouse MAb Validated Applications

Application Dilution
WB his antibody can be used at 1:500-1:1000 with the appropriate secondary reagents to detect H1N1 (A/Puerto Rico/8/1934) HA in WB. Using a DAB detection system, the detection limit for H1N1 (A/Puerto Rico/8/1934) HA is approximately 8 ng/lane under non-reducing conditions.Use of this antibody under reducing conditions is not recommended.
ELISA 1:1000-1:2000
ELISA(Det) 1:1000-1:10000
ELISA(Det): This antibody will detect Influenza H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA in ELISA pair set (Catalog: # SEK11684). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11684-MM05).
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Hemagglutinin/HA Background Information

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Harvey rat sarcoma viral oncogene homolog
  • White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
  • Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
  • Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
  • Lectin microarray analyses reveal host cell-specific glycan profiles of the hemagglutinins of influenza A viruses
    Hiono, T;Matsuda, A;Wagatsuma, T;Okamatsu, M;Sakoda, Y;Kuno, A;

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